| 酪蛋白非磷肽中一种ACE活性抑制肽的分离和鉴定 |
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| 引用本文： | 赵一明,王璋,许时婴,张文斌. 酪蛋白非磷肽中一种ACE活性抑制肽的分离和鉴定[J]. 食品与发酵工业, 2008, 34(4): 21-24 |
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| 作者姓名： | 赵一明 王璋 许时婴 张文斌 |
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| 作者单位： | 江南大学食品学院,江苏无锡,214122 |
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| 基金项目： | 江苏省苏州市科技攻关项目 |
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| 摘 要： | 酪蛋白经碱性蛋白酶Alcalase水解后,加入乙醇提取出的酪蛋白非磷肽(CNPPs)对血管紧张素转化酶(ACE)的活性有很强的抑制作用。采用凝胶过滤色谱Sephadex G-15和反相高效液相色谱(RP—HPLC)对CNPPs进行分离纯化,通过高效液相色谱/电喷雾电离质谱联用分析和氨基酸组成分析鉴定出一种抑制ACE活性肽,其氨基酸序列为Ser-Trp,ACE半抑制浓度为92.8μmol/L。
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| 关 键 词： | 酪蛋白非磷肽 血管紧张素转化酶抑制肽 氨基酸序列 高效液相色谱 电喷雾电离质谱 |
| 修稿时间： | 2007-10-17 |
Isolation and Identification of Inhibitory Peptides for Angiotensin Converting Enzyme in Casein Non-phosphopeptides |
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| Zhao Yiming,Wang Zhang,Xu Shiying,Zhang Wenbin. Isolation and Identification of Inhibitory Peptides for Angiotensin Converting Enzyme in Casein Non-phosphopeptides[J]. Food and Fermentation Industries, 2008, 34(4): 21-24 |
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| Authors: | Zhao Yiming Wang Zhang Xu Shiying Zhang Wenbin |
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| Abstract: | Casein non—phosphopeptides(CNPPs)which showed significant inhibitory activity against the angiotensin converting enzyme(ACE)could be obtained from casein by enzymatic hydrolysis and extracting with ethanol.CNPPs was first isolated by size exclusion chromatography(SEC)Sephadex G-15,and was further purified by reversed-phase HPLC.The pure peptide with ACE inhibitory activity was obtained,the amino acid sequence of which was identified as Ser-Trp by LC/ESI-MS and amino acid composition analysis. And the inhibitory concentration 50%(IC50)was 92.8μmol/L. |
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| Keywords: | Casein non-phosphopeptides inhibitory peptides for angiotensin converting enzyme amino acid sequences high performance liquid chromatography electrospray ionization mass spectrometry |
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