| 紫球藻生长周期可见光吸收光谱与生化变化 |
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| 引用本文： | 王明兹,施巧琴,陈必链,庄惠如,吴松刚. 紫球藻生长周期可见光吸收光谱与生化变化[J]. 食品与发酵工业, 2003, 29(4): 23-26 |
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| 作者姓名： | 王明兹 施巧琴 陈必链 庄惠如 吴松刚 |
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| 作者单位： | 福建师范大学生物工程学院,福州,350007 |
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| 基金项目： | 福建省教育厅资助项目 (No .JA0 1 0 1 60 )，福建省计委资助项目 (No .1 996 0 50 ) |
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| 摘 要： | 测定紫球藻生长周期中活细胞可见光吸收光谱和代谢产物变化。结果表明 ,存在波长为43 0～ 44 5nm、5 5 5～ 5 65nm和 680～ 685nm 3个较大吸收峰 ,还存在 5 0 0～ 5 10nm和 62 0～ 63 0nm 2个次吸收峰。且波长为 44 0、5 60和 680nm时的光吸收值与细胞数成正比增长 ,可用于快速测定紫球藻培养液的细胞浓度
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| 关 键 词： | 紫球藻 生长周期 吸收光谱 细胞浓度 生化组成 |
| 修稿时间： | 2002-08-26 |
Visible Light Absorption Spectrum and Biochemical Change of Porphyridium cruentum in the Growth Circle |
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| Wang Mingzi Shi Qiaoqin Chen Bilian Zhuang Huiru Wu Songgang. Visible Light Absorption Spectrum and Biochemical Change of Porphyridium cruentum in the Growth Circle[J]. Food and Fermentation Industries, 2003, 29(4): 23-26 |
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| Authors: | Wang Mingzi Shi Qiaoqin Chen Bilian Zhuang Huiru Wu Songgang |
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| Abstract: | The visible light absorption spectrum and biochemical compositiion of Porphyridium cr2酰澹睿簦酰? in the growth circle were investigated. The results showed that it had three absorbing peaks at light wavelength of 430～445?nm, 555～565?nm and 680?nm～685?nm,and two sub-absorbing peaks at 500～510?nm and 520～630?nm Moreover,light absorption value at 440?nm,560?nm and 680?nm increased in direct ratio to the cell number This can be a quick method to determining cell concentration of Porphyridium entum during incubation |
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| Keywords: | Porphyridium cruentum growth circle absorption spectrum cell concentration growth index biochemical composition |
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