| 利用LC-MS技术解析黑果枸杞、红果枸杞代谢物的差异 |
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| 引用本文： | 彭玉娇,崔学宇,邵元元,陈众峰,杜潇,曾文萍,崔婷婷.利用LC-MS技术解析黑果枸杞、红果枸杞代谢物的差异[J].食品与生物技术学报,2021,40(9):56-63. |
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| 作者姓名： | 彭玉娇 崔学宇 邵元元 陈众峰 杜潇 曾文萍 崔婷婷 |
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| 作者单位： | 南宁师范大学 广西地标作物大数据工程技术研究中心/北部湾环境演变与资源利用教育部重点实验室/广西地表过程与智能模拟重点实验室，广西 南宁 530001;上海欧易生物医学科技有限公司，上海 201210 |
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| 摘 要： | 为了分析比较黑果枸杞和红果枸杞代谢物的差异。利用液相色谱质谱联用技术(LC-MS)分析其代谢产物,共检测出6 982种代谢物,包括负离子2 704个,正离子4 278个;主成分分析(PCA)分析发现,黑果枸杞和红果枸杞可以被区分开;对差异代谢物的分析发现,二者共有501种差异代谢物,其中表达量前50的差异代谢物中有7种是黄酮类物质,6种是氨基酸和多肽类物质;11个代谢通路被显著富集,其中4个和氨基酸代谢相关。本研究为深入了解枸杞中的氨基酸代谢以及对黑果枸杞和红果枸杞的差异利用提供一定依据。
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| 关 键 词： | 黑果枸杞 红果枸杞 液相色谱质谱联用技术 代谢物 |
Analysis of the Differences of Metabolites between Lycium ruthenicum Murr. and Lycium barbarum L. by LC-MS |
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| PENG Yujiao,CUI Xueyu,SHAO Yuanyuan,CHEN Zhongfeng,DU Xiao,ZENG Wenping,CUI Tingting.Analysis of the Differences of Metabolites between Lycium ruthenicum Murr. and Lycium barbarum L. by LC-MS[J].Journal of Food Science and Biotechnology,2021,40(9):56-63. |
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| Authors: | PENG Yujiao CUI Xueyu SHAO Yuanyuan CHEN Zhongfeng DU Xiao ZENG Wenping CUI Tingting |
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| Affiliation: | Guangxi Geographical Indication Crops Research Center of Big Data Mining and Experimental Engineering Technology/Key Laboratory of Beibu Gulf Environment Change and Resources Utilization of Ministry of Education/Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation, Nanning Normal University, Nanning 530001, China;Shanghai Oebiotech Co., Ltd., Shanghai 201210, China |
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| Abstract: | This study aimed to analyze and compare the differences of metabolites between Lycium ruthenicum< Murr. and Lycium barbarum L.. Liquid chromatography mass spectrometry technique (LC-MS) was adopted to analyze the metabolites. A total of 6 982 metabolites were detected, including 2 704 negative ions and 4 278 positive ions. Principle component analysis (PCA) displayed that Lycium ruthenicum Murr. and Lycium barbarum L. could be distinguished. Analysis of differential metabolites revealed a total of 501 differential metabolites. Among the top 50 differentially expressed metabolites, 7 were flavonoids, and 6 were amino acids and polypeptides. 11 metabolic pathways were significantly enriched, 4 of which were associated with amino acid metabolism. This study provided a basis for in-depth understanding of the biosynthesis of amino acid metabolism and the differential utilization of Lycium ruthenicum Murr. and Lycium barbarum L.. |
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| Keywords: | Lycium ruthenicum Murr Lycium barbarum L LC-MS metabolites |
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