Synthesis of bio-functionalized copolymer particles bearing carboxyl groups via a microfluidic device

Monodisperse copolymer particles carrying surface carboxyl groups in the range of 50–200 μm were prepared by in situ UV polymerization of ethyleneglycol dimethacrylate (EGDMA) with acrylic acid (AA) via a microfluidic flow-focusing device (MFFD). The design of the coaxial orifices in the MFFD enables the confinement of the comonomer liquid thread to the central axis of the microchannel, which can avoid the wetting problem of comonomer liquid with the microchannel and can successfully produce monodisperse copolymer microspheres with coefficient of variance below 5%. The effects of concentration of EGDMA and AA on droplet diameters and the distribution of carboxyl group on particle surfaces were examined. It has been found that, increasing the concentration of AA would decrease particle sizes, but increase the distribution of carboxyl group on particle surfaces. Bioconjugation of the carboxylated copolymer particles with the anti-rabbit IgG–Cy3 conjugates was successfully demonstrated. By increasing the concentration of AA accompanied with decreasing the particle sizes, high efficiency of bioconjugation on carboxylated copolymer particles was achieved. The rapid continuous synthesis of carboxylated copolymer particles via a microfluidic device provides a reliable control of particle sizes and composition for massive production in biotechnological applications.