|
|||||
|
|
| 繁茂膜海绵中产甲壳素酶菌株筛选的研究 | |
| 引用本文： | 朱妍妍,黄剑宇,牟光庆,靳艳.繁茂膜海绵中产甲壳素酶菌株筛选的研究[J].食品研究与开发,2006,27(11):29-31. |
| 作者姓名： | 朱妍妍 黄剑宇 牟光庆 靳艳 |
| 作者单位： | 1. 中科院大连化学物理研究所,辽宁,大连,116023;大连轻工业学院食物与食品工程学院,辽宁,大连,116034 2. 中科院大连化学物理研究所,辽宁,大连,116023 3. 大连轻工业学院食物与食品工程学院,辽宁,大连,116034;东北农业大学食品学院,黑龙江,哈尔滨,150030 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 通过富集培养和初筛，从繁茂膜海绵中分离到108株产甲壳素酶菌株；其中有12株菌产生的透明圈比较明显。结合平板法和酶活力比较法，筛选出1株高酶活力的优良菌株H7，酶活力为0．5415／mL。通过16SrRNA同源序列分析和系统发育树分析，鉴定菌株H7为Pseudoaheromonas属，和P．ganghwensis/DQ011614相似性达到98％。 |
| 关 键 词： | 繁茂膜海绵 甲壳素酶 酶活力 筛选 交替假单胞菌 |
| 收稿时间： | 2006-05-27 |
| 修稿时间： | 2006年5月27日 |
STUDIES ON STRAINS SCREEN OF PRODUCING CHITINASE FROM HYMENIACIDON PERLEVE |
|
| ZHU Yan-yan,HUANG Jian-yu,MU Guang-qing,JIN Yan.STUDIES ON STRAINS SCREEN OF PRODUCING CHITINASE FROM HYMENIACIDON PERLEVE[J].Food Research and Developent,2006,27(11):29-31. | |
| Authors: | ZHU Yan-yan HUANG Jian-yu MU Guang-qing JIN Yan |
| Abstract: | 108 strains of producing chitinase were isolated from Hymettiacidon perleve by enrichment culture and preliminary screening, and there were 12 strains can produce transparent circle obviously. One good strain H7 of producing high enzyme activity was screened by plate process amt enzyme activity comparison method.The enzyme activity of H7 is 0.54 U/mL.H7 was identified as Pseudoalteromaons by analyzing of homologization sequence of 16S rRNA and phylogenetic tree ,The similarity of H7 is 98 % with P, ganghwensis/DQ011614. |
| Keywords: | Hymeniacidon perleve chitinase enzyme activity screen Pseudoalteromonas |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |