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Selection of the methylotrophic yeast Ogataea minuta as a high-producing host for heterologous protein expression
Affiliation:1. Biologics Technology Research Laboratories, Daiichi Sankyo Co., Ltd., 2716-1 Kurakake, Akaiwa, Chiyoda, Gunma 370-0503, Japan;2. Organic Synthesis Department, Natural Product Research Group, Daiichi Sankyo RD Novare Co., Ltd., 1-16-13 Kitakasai, Edogawa-ku, Tokyo 134-8630, Japan;3. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan;1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;2. The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;3. Lab of Industrial Microorganism & Research and Design Center for Polyols, School of Biotechnology, Jiangnan University, Wuxi 214122, China;1. Department of Life and Environmental System Science, Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama, Kanagawa 236-0027, Japan;2. Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan;3. REPROCELL Inc., 3-8-11 Shin-yokohama, Kohoku-ku, Yokohama, Kanagawa 222-0033, Japan;4. Shonan Laboratory, REPROCELL Inc., 2-26-1 Muraoka-higashi, Fujisawa, Kanagawa 251-8555, Japan;1. College of Biotechnology Engineering, Sichuan University of Science and Engineering, Yibin 644000, China;2. Forgood Distillery Industry Co. Ltd., Mianyang 621000, China;3. Sichuan Taiyuanjing Vinegar Co. Ltd., Zigong 643000, China;1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China;2. Key Laboratory of Agro-Products Primary Processing, Academy of Agricultural Planning and Engineering, MARA, Beijing 100125, China;1. State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, No. 130 Meilong Road, Xuhui District, Shanghai 200237, People’s Republic of China;2. Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, No. 279 Zhouzhu Road, Pudong New Area, Shanghai 201318, People’s Republic of China;3. Research Laboratory for Functional Nanomaterial, National Engineering Research Center for Nanotechnology, No. 28 Jiangchuan East Road, Minhang District, Shanghai 200241, People’s Republic of China;4. School of Materials Science and Engineering, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Minhang District, Shanghai 200240, People’s Republic of China;5. Weigao Shanghai R&D Center, No. 720 Cailun Road, Pudong New Area, Shanghai 201203, People’s Republic of China;1. Kirishima Shuzo Co., Ltd., 4-28-1 Shimokawahigashi, Miyakonojo, Miyazaki 885-8588, Japan;2. The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan;3. MY Food Development Research Institute, 109-3 ichimanjyo, Miyakonojo, Miyazaki 885-0041, Japan
Abstract:
Keywords:Protein expression  Methylotrophic yeast  Taxonomy  Monocolony isolation
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