The effect of high hydrostatic pressure on the flow behaviour of skim milk–gelatin mixtures |
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Authors: | Yacine Hemar Li Hui Liu Nicolas Meunier Brad W Woonton |
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Affiliation: | 1. Faculty of Mechanical Engineering, University of Tabriz, Tabriz, Iran;2. Department of Mechanical Engineering, Sharif University of Technology, Tehran, Iran;1. Department of Wine Food and Molecular Biosciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, Christchurch, New Zealand;2. Department of Food Sciences, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand |
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Abstract: | The flow behaviour of aqueous solutions of gelatin, and skim milk–gelatin mixtures treated by high-pressure processing (HPP) were investigated. HPP was carried out at 5 °C for 15 min, at 150 MPa, 300 MPa, 450 MPa and 600 MPa, and the gelatin concentrations were varied from 0 to 1 wt.%. Viscosity measurements showed that the HPP treatment did not affect the flow behaviour of gelatin alone, nor that of the skim milk–gelatin mixtures made with < 0.4 wt.% gelatin. However, at gelatin concentration > 0.4 wt.%, the mixtures treated with 300 and 450 MPa exhibited a peculiar flow behaviour, where at intermediate shear rates the viscosity was higher than that of the non-treated mixture or the mixtures treated at 150 MPa and 600 MPa. Particle size measurements showed that for gelled mixtures (> 0.4 wt.% gelatin) 300 MPa HPP treatment resulted in an increase in the particle size, while at all other pressure treatments (> 150 MPa), a shift in particle size distribution to lower sizes was observed. Confocal microscopy showed that these skim milk–gelatin mixtures were phase-separated with a gelatin continuous phase, this was confirmed by dynamic rheological measurements which showed that qualitatively the viscoelastic properties of the mixtures were the same. A mechanism of the effect of high-pressure treatment on the casein micelle in skim milk–gelatin mixtures is proposed.Industrial relevanceThis fundamental work, dealing with the effect of high pressure on the physicochemical properties skim milk–gelatin mixtures could be relevant to the industry in several ways. Firstly, skim milk–gelatin mixtures are widely used in the dairy industry, particularly in yoghurt manufacture, where gelatine is used as a stabiliser. In addition the application of High Hydrostatic Pressure to such a system is also relevant, as this technology could be used as a substitute to the conventional heat treatment processes. Secondly, an important finding of this study is that under certain conditions of high pressure and gelatine concentration, an increase in viscosity is observed at intermediate shear-rate (between 10 and 100 s?1). This is highly relevant to Industry if the system requires subsequent pumping. Thirdly, from a sensory view point, this range of shear rates (10 and 100 s?1) is comparable to that experienced by a food bolus during swallowing. Thus, this effect of high pressure on the viscosity can influence sensory attribute of the skim milk–gelatin food system. |
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