Characteristic features of InfA-15 monoclonal antibody recognizing H1, H3, and H5 subtypes of hemagglutinin of influenza virus A type |
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| Authors: | Emi Hifumi Naoko Fujimoto Kazuya Ishida Hirokazu Kawawaki Taizo Uda |
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| Affiliation: | 1. Research Center for Applied Medical Engineering, Oita University, 700 Dannoharu, Oita-shi, Oita 870-1192, Japan;2. Applied Biochemistry, Faculty of Engineering, Oita University, 700 Dannoharu, Oita-shi, Oita 870-1192, Japan;3. PRESTO, Japan Science and Technology Corporation (JST), Kawaguchi, Saitama 332-0012, Japan.;1. Centre for Biologics Evaluation, Biologics and Genetic Therapies Directorate, Health Canada and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Ottawa, ON, Canada;2. Canadian Food Inspection Agency, Ottawa, ON, Canada;3. Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia;4. Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia;5. National Institutes for the Control of Food and Drug and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, PR China;6. National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada;7. Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada;1. Department of Physics and Astronomy, The University of Western Ontario, London, Ontario N6A 3K7, Canada;2. Department of Earth, Planetary and Space Sciences, University of California at Los Angeles, 595 Charles Young Drive East, Los Angeles, CA 90095-1567, USA;3. Centre for Planetary Science and Exploration, The University of Western Ontario, London, Ontario N6A 5B8, Canada;4. No. 1 Senior High School of Ürumqi, Ürumqi, Xinjiang, China;1. Department of Physiology and Research Center of Transport Protein for Medical Innovation, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand;2. Department of Chemistry and Center for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand;1. Department of Chemistry, The Chinese University of Hong Kong, Shatin N. T., Hong Kong;2. Hefei National Laboratory for Physical Sciences at Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China;1. The State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, Fourth Military Medical University, 169 Changle West Road, Xi''an 710032, China;2. The State Key Laboratory of Cancer Biology, Department of Immunology, Fourth Military Medical University, 169 Changle West Road, Xi''an 710032, China;3. Clinical Laboratory, 451 Hospital of Chinese People''s Liberation Army, 269 Youyi East Road, Xi''an 710054, China;4. Department of Cardiology, Xijing Hospital, Fourth Military Medical University, 15 Changle West Road, Xi''an 710032, China;5. School of Aerospace Medicine, Fourth Military Medical University, 169 Changle West Road, Xi''an 710032, China;6. Research Center for Clinical Molecular Biology, Xijing Hospital, Fourth Military Medical University, 15 Changle West Road, Xi''an 710032, China;7. Department of Molecular Microbiology and Immunology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA;1. Medical Life Sciences Institute, Department of Medical Sciences, Ministry of Public Health, Muang, Nonthaburi, Thailand;2. National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Muang, Nonthaburi, Thailand;3. Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan;4. Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University, Kanonji, Kagawa, Japan;5. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan;6. Ina Laboratory, Medical & Biological Laboratories Corporation, Ltd., Ina, Nagano, Japan;7. Food and Drug Administration, Ministry of Public Health, Muang, Nonthaburi, Thailand;8. Japan Science and Technology Agency/Japan International Cooperation Agency, Science and Technology Research Partnership for Sustainable Development (JST/JICA, SATREPS), Tokyo, Japan |
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| Abstract: | Hemagglutinin molecule is an envelope protein of influenza virus and plays an important role in the infection to human cells. Many mutations are observed in the molecule, which generates sixteen subtypes (H1–H16) of the hemagglutinin molecule for influenza virus A type. The subtypes such as H1, H2, H3, and H5 out of the sixteen are underlined molecules, which are responsible to Spain, Asia, Hong Kong, and Avian Flu, respectively. Based on the sequence analysis, three short sequences, which are highly conserved in the subtypes of influenza virus A type, were extracted. The sequence peptides were chemically synthesized and conjugated with BSA for immunization into Balb/c mice. A sequence GMVDGWYG located at the domain of fusion protein in the hemagglutinin molecule exhibited a high immuno-response, resulting in the production of a monoclonal antibody (mAb; InfA-15). The unique features of InfA-15 mAb were investigated from the viewpoint of immunological reaction, the binding affinity, the steric conformation, etc. The InfA-15 mAb could react with the H1, H3, and H5 subtype of hemagglutinin molecule of influenza virus A type. ELISAs using InfA-15 mAb suggested a wide reaction spectrum for the hemagglutinin of many important influenza viruses A type. |
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