| 富硒乳酸菌的筛选及鉴定 |
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| 引用本文： | 曾议霆,郭溪浪,周康,刘书亮,韩新锋.富硒乳酸菌的筛选及鉴定[J].食品科学,2015,36(3):178-182. |
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| 作者姓名： | 曾议霆 郭溪浪 周康 刘书亮 韩新锋 |
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| 作者单位： | 四川农业大学食品学院，四川 雅安 625014 |
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| 摘 要： | 对5 种15 株乳酸菌的生长曲线进行了测定，并对亚硒酸钠添加质量浓度、耐硒和富硒能力进行比较，筛选出了富硒优势乳酸菌，并进行了生理生化和分子鉴定。结果表明：适宜的加硒时间为培养后的第6小时，培养时间为24 h。通过对9 株菌株比较，确定了较适宜的亚硒酸钠添加质量浓度为6 μg/mL。最终筛选出BC-25为富硒优势菌株，富硒量425.79 μg/g，硒转化率达27.00%，经16S rDNA分析鉴定其为植物乳杆菌（Lactobacillus plantaru）。
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| 关 键 词： | 乳酸菌 富硒 筛选 鉴定 |
Screening and Identification of Se-Enriching Lactic Acid Bacteria |
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| ZENG Yiting;GUO Xilang;ZHOU Kang;LIU Shuliang;HAN Xinfeng.Screening and Identification of Se-Enriching Lactic Acid Bacteria[J].Food Science,2015,36(3):178-182. |
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| Authors: | ZENG Yiting;GUO Xilang;ZHOU Kang;LIU Shuliang;HAN Xinfeng |
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| Affiliation: | College of Food Science, Sichuan Agricultural University, Ya’an 625014, China |
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| Abstract: | A total of 15 lactic acid bacteria derived from 5 species were studied by comparing their growth curves and
abilities to tolerate and enrich Se. We also identified and characterized the selected strain by biochemical and molecular
biological methods. The results showed that the optimum time to add Se was at the end of 6-hour culture period after which
the culture was carried out for another 18 hours. The optimum sodium selenite concentration for Se enrichment was found to
be 6 μg/mL by comparing 9 selected strains. The strain BC-25 was eventually selected as the best Se-enriching strain, which
could enrich 425.79 μg/g of Se with a transformation rate of 27.00%. The 16S rDNA sequence results indicated that BC-25
is Lactobacillus plantarum. |
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| Keywords: | lactic acid bacteria Se enrichment screening identification |
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