| 耐促溶剂雷斯青霉菌株N~+注入诱变选育 |
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| 引用本文： | 刘莎,毛淑红,胡晓杰,周瑶,路福平.耐促溶剂雷斯青霉菌株N~+注入诱变选育[J].辐射研究与辐射工艺学报,2012(2):97-102. |
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| 作者姓名： | 刘莎 毛淑红 胡晓杰 周瑶 路福平 |
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| 作者单位： | 天津科技大学生物工程学院,天津300457 |
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| 基金项目： | 国家863项目（2011AA02A211）; 天津市高等学校科技发展基金计划项目（20100601）; 天津科技大学人才引进基金（20080436）资助 |
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| 摘 要： | 在甾体生物催化过程中,向发酵液中添加亲水性有机溶剂,是增加底物溶解进而提高转化效率的有效手段,但有机溶剂对于菌体普遍具有毒性。为此采用N+注入诱变方法,对出发菌株雷斯青霉p-10-8进行改良,获得耐促溶剂菌株60 A-5-8,当投料量增大到4‰时转化率达82.61%。经过5次传代实验证明,该菌株具有较高的遗传稳定性。
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| 关 键 词： | 生物催化 雷斯青霉 离子注入 |
Screening of organic solvent tolerant Penicillium raistrickii induced by ion irradiation |
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| LIU Sha,MAO Shuhong,HU Xiaojie,ZHOU Yao,LU Fuping.Screening of organic solvent tolerant Penicillium raistrickii induced by ion irradiation[J].Journal of Radiation Research and Radiation Processing,2012(2):97-102. |
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| Authors: | LIU Sha MAO Shuhong HU Xiaojie ZHOU Yao LU Fuping |
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| Affiliation: | (The College of Biotechnology, Tianjin University of Science ＆ Technology, Tianjin 300457, China) |
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| Abstract: | In the steroid biocatalytic process, adding hydrophilic organic solvent to the fermentation medium is an effective method to increase the substrate solubility and the conversion efficiency, but organic solvents are often toxic to mycelium. To solve this problem, the Penicillium raistrickii p-10-8 was mutated by N＋ ion implantation, and an organic solvent tolerant strain named 60 A-5-8 was obtained, of which the conversion rate reached 82.61% when the amount of substrate increase 4%0. In addition, the mutant strain shows high genetic stability even after 5 generations of cultivation. |
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| Keywords: | Biocatalysis Penicillium raistrickii Ion implantation |
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