Plant flavonol quercetin and isoflavone biochanin A differentially induce protection against oxidative stress and inflammation in ARPE-19 cells |
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Authors: | Niina MM SavirantaLaura Veeroos Lars J GranlundViivi H Hassinen Kai KaarnirantaReijo O Karjalainen |
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Affiliation: | a University of Eastern Finland, Department of Biosciences, P.O. Box 1627, 70211 Kuopio, Finlandb University of Eastern Finland and Kuopio University Hospital, Department of Ophthalmology, P.O. Box 1627, 70211 Kuopio, Finlandc AgriFood Research Finland, 31600 Jokioinen, Finland |
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Abstract: | In this work, differential ability of plant flavonol quercetin and plant isoflavone biochanin A to modulate oxidative stress and inhibit inflammation-related responses was investigated using human retinal pigment epithelial cells (RPE) at gene expression level. Quercetin protected cells from oxidative stress-induced cell death, whereas biochanin A had no statistically significant protective effects. Quercetin reduced the expression of cytokines IL-6 and IL-1?? in cells treated with H2O2, and expression levels of Nrf2 and HO-1 were increased by quercetin treatment suggesting protective function against oxidative stress. Our data indicate that quercetin may protect cells by inhibiting the production of pro-inflammatory factors such as IL-6, and by inducing the expression of ROS-catalyzing phase II proteins such as HO-1. Therefore, plant extracts rich in flavonol quercetin may be an interesting resource for functional food products and other foods targeted for reduced risks of age-related macular degeneration. |
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Keywords: | AMD age-related macular degeneration ARE antioxidant response element COX-2 cyclooxygenase-2 GST glutathione S-transferase HO-1 heme oxygenase-1 IL-1?? interleukin-1?? IL-6 interleukin-6 iNOS inducible nitric oxide synthase NF-??B nuclear factor-??B Nrf2 nuclear factor-erythroid 2-related factor-2 qRT-PCR quantitative real-time polymerase chain reaction ROS reactive oxygen species TNF-?? tumor necrosis factor ?? |
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