Dilute oxalic acid pretreatment for biorefining giant reed (Arundo donax L.) |
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Authors: | Danilo Scordia Salvatore L CosentinoJae-Won Lee Thomas W Jeffries |
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Affiliation: | a Dipartimento di Scienze delle Produzioni Agrarie e Alimentari-DISPA, University of Catania, Via Valdisavoia 5, 95123 Catania, Italy b Departmentof Forest Products and Technology (BK 21 Program), Chonnam National University, Gwangju 500-757, South Korea c Bioenergy Research Center, Chonnam National University, Gwangju 500-757, South Korea d US Department of Agriculture, Forest Service, Forest Products Laboratory, One Gifford Pinchot Drive, Madison, WI, USA e Department of Bacteriology, University of Wisconsin-Madison, 420 Henry Mall, Madison, WI 53706, USA |
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Abstract: | Biomass pretreatment is essential to overcome recalcitrance of lignocellulose for ethanol production. In the present study we pretreated giant reed (Arundo donax L.), a perennial, rhizomatous lignocellulosic grass with dilute oxalic acid. The effects of temperature (170-190 °C), acid loading (2-10% w/w) and reaction time (15-40 min) were handled as a single parameter, combined severity. We explored the change in hemicellulose, cellulose and lignin composition following pretreatment and glucan conversion after enzymatic hydrolysis of the solid residue. Two different yeast strains, Scheffersomyces (Pichia) stipitis CBS 6054, which is a native xylose and cellobiose fermenter, and Saccharomyces carlsbergensis FPL-450, which does not ferment xylose or cellobiose, were used along with commercial cellulolytic enzymes in simultaneous saccharification and fermentation (SSF). S. carlsbergensis attained a maximum ethanol concentration of 15.9 g/l after 48 h at pH 5.0, while S. stipitis, at the same condition, took 96 h to reach a similar ethanol value; increasing the pH to 6.0 reduced the S. stipitis lag phase and attained 18.0 g/l of ethanol within 72 h. |
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Keywords: | Arundo donax Xylan Cellulose Ethanol SSF Fractionation |
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