Phenolics and antioxidant activity of lentil and pea hulls |
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Authors: | B. Dave Oomah,Franç ois CasparLinda J. Malcolmson,Anne-Sophie Bellido |
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Affiliation: | a National Bioproducts and Bioprocesses Program, Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, 4200 Highway 97, P.O. Box 5000, Summerland, BC, Canada V0H 1Z0b Université de la Réunion, Ecole Supérieure d''Ingénieurs en Développement Agroalimentaire Intégré (ESIDAI), 97490 Sainte Clotilde, Ile de la Réunion, Francec Special Crops and Pulses, Canadian International Grains Institute (CIGI), 1000-303 Main Street, Winnipeg, Manitoba, Canada R3C 3G |
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Abstract: | Hulls obtained by pilot-scale dehulling process from green and red lentils and yellow peas were fractionated into hulls (> 500 ??m) and residues (< 500 ??m). These fractions together with the corresponding whole seeds were extracted with four solvents, aqueous (70%) acetone, (80%) ethanol, hot water (70-80 °C) and water (22 ± 1 °C) and evaluated for antioxidant activity in relation to phenolic contents. Aqueous acetone (70%, v/v) extracted the highest level of total phenolics at about 87 mg of catechin equivalent per gram of sample from lentil hulls followed by hot water, water and aqueous ethanol (80%, v/v). Red lentil hull with maximum concentration of phenolic compounds exhibited the strongest antioxidant activity of 260 mg (1040 ??M) trolox equivalent/g hull. Hot water and water extracted significantly higher total phenolics from whole pulse and their residue than other solvents, but with weaker antioxidant activity. Aqueous ethanol was always the best extractant of phenolic antioxidant from yellow pea irrespective of its millstream. The most potent phenolic antioxidant was obtained from water extract of green and red lentil hulls, followed by those extracted with ethanol, acetone, and hot water. Total phenolic content was highly correlated with antioxidant activity of pulses and its millstreams. |
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Keywords: | Pulse hulls Lentils Yellow pea Antioxidant activity Solvent extraction Phenolics Water extract Hulls |
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