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Influence of C/P and C/N ratios and microbial characterization in hydrogen and ethanol production in an anaerobic fluidized bed reactor
Authors:Mariana Fronja Carosia  Cristiane Marques dos Reis  Isabel Kimiko Sakamoto  Maria Bernadete Amâncio Varesche  Edson Luiz Silva
Affiliation:1. Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. João Dagnone, 1100, Jd. Santa Angelina, CEP 13563-120, São Carlos, SP, Brazil;2. Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luis, Km 235, CEP 13565-905, São Carlos, SP, Brazil;3. Department of Industrial Chemistry, State University of Mato Grosso do Sul, Rodovia, CEP 79804-970, Dourados, MS, Brazil
Abstract:The aim of this study was to evaluate the influence of C/P and C/N ratios on the production of hydrogen and ethanol in four anaerobic fluidized bed reactors: R1 (C/N = 100), R2 (C/N = 150), R3 (C/N = 200), and R4 (C/N = 250). The hydraulic retention time (HRT) was maintained at 2 h, and the C/P ratios varied from 300 to 1100. Reactors were filled with grounded tire and fed with synthetic substrate containing glucose (5000 mg L?1). The effluent pH was around 3.7. The highest values for hydrogen yield (HY) and hydrogen production rate (HPR) were obtained at a C/P = 700 ratio in all reactors. The best performance was achieved at R3 (C/N = 200): HY of 0.76 mol H2 mol?1 glucose and HPR of 0.70 L h?1 L?1. The highest value for ethanol yield was obtained at C/P = 700 in R1 (1.5 mol EtOH mol?1 glucose). Ethanol- and hydrogen-producing fermenters, such as Ethanoligenens sp. and Clostridium sp. were identified by molecular analysis. Lactobacillus sp. was also identified in this study.
Keywords:Nitrogen  Phosphorus  Grounded tire
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