The influence of organ,season and drying method on chemical composition and antioxidant and antimicrobial activities of Juniperus phoenicea L. essential oils |
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Authors: | Monia Ennajar Jalloul Bouajila Ahmed Lebrihi Florence Mathieu Arlette Savagnac Manef Abderraba Aly Raies Mehrez Romdhane |
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Affiliation: | 1. Laboratoire de Physicochimie des Matériaux, IPEST, BP51, La Marsa 2070, Tunisia;2. Unité de Modélisation, Analyse et commande des systèmes, Ecole Nationale d'Ingénieur, 6029 Gabés, Tunisia;3. Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique UMR CNRS 5623, Université Paul‐Sabatier, F‐31062 Toulouse, France;4. Université de Toulouse, LGC UMR 5503 (CNRS/INPT/UPS), ENSAT/INPT, BP 32 607, Auzeville‐Tolosane, 31326 Castanet‐Tolosan, France;5. UFR SVT, Université Paul‐Sabatier, F‐31062 Toulouse, France;6. LMBA. Faculté des Sciences de Tunis. Campus Universitaire 2092 El Manar II, Tunisia |
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Abstract: | BACKGROUND: Juniperus phoenicea is an important medicinal plant. In the present study, essential oils (18 samples) from leaves and berries of Juniperus phoenicea L. (Cupressaceae), obtained by various drying methods and in different collection months, were analysed by gas chromatography–mass spectrometry and also evaluated for in vitro antimicrobial and antioxidant activities. Correlations were studied between antimicrobial activity and the chemical composition of essential oils. RESULTS: Sixty‐seven compounds were identified in essential oils, representing 97.7–100%. Essential oils were dominated by monoterpenes and sesquiterpenes, which presented 35.0–93.3% and 6.7–62.0%, respectively, depending of organ, season and drying method. Antimicrobial tests showed that essential oils strongly inhibited the growth of Gram‐positive microorganisms and Mucor ramamnianus, but was inactive against Gram‐negative strains. Antioxidant activity was tested using the ABTS radical‐scavenging assay. Most samples showed good activity (the best IC50 = 41.7 ± 1.5 mg L?1). CONCLUSIONS: It could be concluded that drying of leaves of J. phoenicea in the sun and berries in oven‐drying was more suitable and was recommended for obtaining higher essential oil yield, but for a higher percentage of some special components such as α‐pinene and δ‐3‐carene shade‐drying was more suitable. Copyright © 2009 Society of Chemical Industry |
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Keywords: | Juniperus phoenicea L (Cupressaceae) essential oil GC‐MS antimicrobial activity antioxidant activity ABTS |
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