Structural investigation of bacterial lipopolysaccharides by mass spectrometry and tandem mass spectrometry |
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| Authors: | Joseph H. Banoub Anas El Aneed Alejandro M. Cohen Nicolas Joly |
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| Affiliation: | 1. Fisheries and Oceans Canada, Science Branch, Special Projects, P.O. Box 5667, St. John's, Newfoundland, Canada A1C 5X1;2. Department of Chemistry, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3V6;3. College of Pharmacy and Nutrition, University of Saskatchewan, Thorvaldson Building, 110 Science Place, Saskatoon, Saskatchewan, Canada S7N 5C9;4. Unité de Catalyse et de Chimie du Solide, Site de l'Artois—UMR CNRS 8181, I.U.T. de Béthune, Département Chimie, 1230 rue de l'Université, BP819, 62408 Béthune Cedex, France |
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| Abstract: | Mass spectrometric studies are now playing a leading role in the elucidation of lipopolysaccharide (LPS) structures through the characterization of antigenic polysaccharides, core oligosaccharides and lipid A components including LPS genetic modifications. The conventional MS and MS/MS analyses together with CID fragmentation provide additional structural information complementary to the previous analytical experiments, and thus contribute to an integrated strategy for the simultaneous characterization and correct sequencing of the carbohydrate moiety. © 2010 Wiley Periodicals, Inc., Mass Spec Rev 29:606–650, 2010 |
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| Keywords: | MS and MS/MS analyses O‐antigen LPS core oligosaccharide lipid A |
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