Antioxidant and antibacterial activities of various seabuckthorn (Hippophae rhamnoides L.) seed extracts |
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| Affiliation: | 1. Human Resource Development Department, Central Food Technological Research Institute, Mysore 570 020, India;2. Fruit and Vegetable Technology Department, Central Food Technological Research Institute, Mysore 570 020, India;1. Department of Anesthesiology, Investigational Intensive Care Unit, The University of Texas Medical Branch and Shriners Burns Hospital for Children, 601 Harborside Drive, Galveston, TX 77555-1102, USA;2. Department of Plastic and Reconstructive Surgery, Tokyo Women''s Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan;3. Center for Electromechanics, University of Texas, 1 University Station PRC R7000, Austin, TX 78712, USA;1. Defence Institute of High Altitude Research, DRDO, Leh, Jammu and Kashmir 194101, India;2. Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh 173215, India;1. Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, University of Lodz, Poland;2. Laboratory of Microbiological and Technical Services, Faculty of Biology and Environmental Protection, University of Lodz, Poland;3. Institute of Soil Science and Plant Cultivation, State Research Institute, Pulawy, Poland;1. Defence Institute of High Altitude Research (DIHAR), DRDO, Ministry of Defence, C/o 56 APO, Leh-Ladakh 901205, India;2. Department of Physiology, All India Institute of Medical Sciences (AIIMS), Bhubaneswar, India |
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| Abstract: | Seabuckthorn (Hippophae rhamnoides L.) seeds were successively extracted with chloroform, ethyl acetate, acetone and methanol (MeOH) using a Soxhlet extractor for 8 h each. The crude extracts were screened for antioxidant and antibacterial activities. The reducing power and antioxidant activities evaluated in various in vitro models (1,1-diphenyl-2-picrylhydrazine and liposome model system) showed the highest activity for MeOH extract. The MeOH extract was also found to posses maximum antibacterial activity. The MIC values, with respect to MeOH extract for Bacillus cereus, Bacillus coagulans, Bacillus subtilis, Listeria monocytogenes, Yersinia enterocolitica, were found to be 200, 300, 300, 300, and 350 ppm, respectively. These results indicated the possibility of using seabuckthorn seeds for medicinal uses and food preservation. |
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