In vitro antioxidant activities of the methanol extracts of five Allium species from Turkey |
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| Affiliation: | 1. Department of Biology, Faculty of Science and Literature, Cumhuriyet University, 58140 Sivas, Turkey;2. Department of Chemistry, Faculty of Science and Literature, 58140 Sivas, Turkey;1. Research Team of Cellular Genomics and Molecular Techniques of Investigations, Department of Biology, Faculty of Sciences, University of Moulay Ismail, B.P. 11201 Zitoune Meknès, Morocco;2. UCIBIO.REQUIMTE, Laboratory of Biochemistry, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal;3. LAQV.REQUIMTE, Laboratory of Bromatology and Hydrology, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal;1. Departamento de Producción Animal, Universidad de León, León, Spain;2. Instituto de Ganadería de Montaña (CSIC-ULE), León, Spain;3. Departamento de Producción Agraria, Universidad Politécnica de Madrid, Madrid, Spain;1. Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya str., 167982 Syktyvkar, Russia;2. Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, 159, Prospect 100-letija, 690022 Vladivostok, Russia;3. Far Eastern Federal University, 8, Sukhanova str., 690022 Vladivostok, Russia |
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| Abstract: | This study was designed to examine the in vitro antioxidant activities of the methanol extracts of five Allium species, namely Allium nevsehirense, A. sivasicum, A. dictyoprosum, A. scrodoprosum subsp. rotundum and A. atroviolaceum; the former two are endemic for the Turkish flora. The extracts were screened for their possible antioxidant activities by two complementary tests; DPPH free radical-scavenging and β-carotene/linoleic acid assays. In the first case, non-polar subfractions of the extracts did not show any antioxidant potential, while the polar subfractions exhibited marked activity. Among the polar ones, the most active one was A. atroviolaceum with an IC50 of 79.0 ± 2.75 μg/ml. In the β-carotene/linoleic acid assay, the inhibition ratios of the oxidation of linoleic acid by A. atroviolaceum and A. dictyoprosum were too close to each other (71.2 ± 2.20% and 72.3 ± 1.20%, respectively), while that of the synthetic antioxidant, BHT, was 96%. |
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