Antioxidant activity of brown pigment and extracts from black sesame seed (Sesamum indicum L.) |
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| Affiliation: | 1. Key Laboratory of Food Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China;2. Department of Biology, Changshu College of Science and Technology, Changshu 215500, PR China;1. Ilie Murgulescu Institute of Physical Chemistry, Romanian Academy, 202 Splaiul Independentei, 060021, Bucharest, Romania;2. Politehnica University of Bucharest, Faculty of Chemistry, 1-7 Polizu Street, 011061, Bucharest, Romania;3. National Institute for Chemical Pharmaceutical Research and Development, 112 Calea Vitan, 031299, Bucharest, Romania;4. University of Bucharest, Faculty of Biology, Microbiology Department, Research Institute of the University of Bucharest-ICUB, Life, Environmental and Earth Sciences Division, 91-95 Splaiul Independentei, Bucharest, Romania;5. Stefan S. Nicolau Institute of Virology, Cellular and Molecular Pathology Department, 285 Mihai Bravu Avenue, Bucharest, Romania;1. Département de Biologie, Unité Analyses Alimentaires, Ecole Nationale d’Ingénieurs de Sfax, Route de Soukra, 3038 Sfax, Tunisia;2. Research Associate, Missouri Botanical Garden, St. Louis, MO, USA;3. Confiserie TRIKI-Le Moulin, Route de Gabes, 3052 Sfax, Tunisia;4. Unité de Technologie des Industries Agro-alimentaires, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des déportés, 2, 5030 Gembloux, Belgium |
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| Abstract: | The antioxidant activities of brown pigment, extract of n-hexane and extract of supercritical carbon dioxide extraction of black sesame seeds were investigated in this study. Kinetics of anti-radical activity showed that the reaction between DPPH and brown pigment of sesame seed was rapid and reached the steady state in 10 min. Extracts from supercritical carbon dioxide extraction and n-hexane extraction reacted with DPPH slowly and the absorbance became stable after 35 min. However, α-tocopherol and trolox were rapid while the kinetic behaviour of BHA was intermediate. The brown pigment of sesame seed also showed a lower EC50 (13.5 μg ml?1) than the other two extracts and α-tocopherol, which was about 7–10 fold of the antioxidant activity of the supercritical carbon dioxide extract and the n-hexane extract. The ferric thiocyanate (FTC) method also showed that the brown pigment of sesame seed provided higher inhibition activity against lipid peroxidation at 200 μg ml?1 than did the supercritical carbon dioxide extract an n-hexane extract at 1 mg ml?1. However, the activities of the supercritical carbon dioxide extract and n-hexane extracts were significantly higher than that of α-tocopherol. In the linoleic acid system, the brown pigment of black sesame seed showed an equal antioxidant activity to BHA and higher than trolox and α-tocopherol. The results indicated that the brown pigment of sesame seed possessed excellent antioxidant activity. |
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