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Phenols,proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities
Affiliation:1. Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova 17, SI-2000 Maribor, Slovenia;2. VITIVA d.o.o., Nova vas pri Markovcih 98, SI-2281 Markovci, Slovenia;1. University of Maribor, Faculty of Medicine, Institute of Biomedical Sciences, Taborska ulica 8, SI-2000 Maribor, Slovenia;2. University of Maribor, Faculty of Medicine, Department of Pharmacology, Taborska ulica 8, SI-2000 Maribor, Slovenia;3. University of Maribor, Faculty of Chemistry and Chemical Engineering, Laboratory for Analytical Chemistry and Industrial Analysis, Smetanova ulica 17, SI-2000 Maribor, Slovenia;4. University of Tirana, Faculty of Natural Sciences, Boulevard “Zogu I”, AL-1001 Tirana, Albania;5. University of Nova Gorica, The Laboratory for Environmental and Life Sciences, Vipavska 13, SI-5001 Nova Gorica, Slovenia;1. University of Novi Sad, Serbia;2. Tubitak Marmara Research Centre, Food Institute, P.O. Box 21, 41470 Gebze, Kocaeli, Turkey;3. Tubitak Marmara Research Centre, Environment and Cleaner Production Institute, P.O. Box 21, 41470 Gebze, Kocaeli, Turkey;1. Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), Espinardo, Murcia, Spain;2. Faculty of Engineering, Food Engineering Program, Corporación Universitaria Lasallista, Caldas-Antioquia, Colombia;3. Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-University of Montpellier – ENSCM, Montpellier, France;1. College of Chemistry and Chemical Engineering, Henan Polytechnic University, Jiaozuo 454003, China;2. College of Science and Technology, Jiaozuo Teachers College, Jiaozuo, 454000, China
Abstract:Methanol extracts prepared from five plant materials native to the Mediterranean area, namely olive tree (Olea europaea) leaf, St. John's wort (Hypericum perforatum), hawthorn (Crataegus laevigata), oregano (Origanum vulgare) and laurel leaf (Lauris nobilis), were examined for their phenolic components. Total phenolic content was determined by the Folin–Ciocalteu method. The content of proanthocyanidins in acid-hydrolysed extracts was determined spectrophotometrically. The contents of free flavones (apigenin and luteolin) and flavonols (kaempferol, myricetin and quercetin) were determined by HPLC analysis. The time of hydrolysis of flavones, flavonols and proanthocyanidins was optimised.Antioxidant activities of apigenin, luteolin, kaempferol, myricetin, quercetin and of plant extracts were examined. Antioxidative activities were studied in sunflower oil at 98 °C, by measuring peroxide value, and in an aqueous emulsion system of β-carotene and linoleic acid by measuring the absorbance of the sample. Among flavones and flavonols investigated, only myricetin inhibited oxidation of sunflower oil. All other flavones and flavonols showed pro-oxidative activity. Oppositely, in the emulsion system, only apigenin showed pro-oxidative activity while other flavones and flavonols and plant extracts inhibited oxidation of β-carotene.
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