荧光假单胞菌AR4在2-酮基-D-葡萄糖酸工业生产中的应用研究

在噬菌体存在的情况下,采用抗噬菌体菌株荧光假单胞菌(Pseudomonasfluorescens)AR4在200m3发酵罐上进行了2-酮基-D-葡萄糖酸的发酵生产。结果表明,荧光假单胞菌AR4对生产环境中噬菌体的抗性稳定,发酵周期短,发酵转化率高;经过10次传代,荧光假单胞菌AR4对噬菌体的抗性及其发酵生产2-酮基-D-葡萄糖酸的能力没有改变;该菌株的发酵产物可以用于食品抗氧化剂D-异抗坏血酸钠的合成。

Study on the Application of Phage-resistant Strain Pseudomonas fluorescens AR4 in Industrial Production of 2-keto-D-gluconic Acid

In the presence of phages, 2-keto-D-gluconic acid fermentation by Pseudomonasfluorescens AR4, which was a phageresistant mutant obtained from the mutation of the strain A46, was conducted in 200m^3 fermentor. The results showed that the mutant resistance against the phages was stable, with shorter fermentation period and higher fermentation conversion rate than its parent. After 10 generations, its resistance against the phages and 2-keto-D-gluconic acid producing ability had not changed. The fermentation product of P. fluorescens AR4 could be used in the synthesis of sodium D-isoascorbate.