Characterization of cellulase under various intensities of static magnetic fields |
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| Authors: | Jingyu Ran Shaoyi Jia Yong Liu Songhai Wu |
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| Affiliation: | aSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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| Abstract: | The activity and secondary conformation of cellulase were investigated under static magnetic fields (0.15, 0.30, and 0.45 T). Compared to control group, activity and conformational change of cellulase were observed by means of UV spectrophotometer and far-UV circular dichroism detection after exposed in various static magnetic fields. The cellulase exhibited its maximal specific activity in the pH range of 4.0–4.6, and the optimal temperature was 60 °C. In addition, the circular dichroism spectra and kinetic parameters of cellulase have also been changed by magnetic fields. |
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| Keywords: | Static magnetic field Cellulase Activity Magnetic intensity Secondary conformation |
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