A31P nuclear magnetic resonance investigation of acyl group transfer from phosphatidylcholine to yield lysophosphatidylcholine in human plasma |
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Authors: | M Hossein Nouri-Sorkhabi David R Sullivan David C Roberts Philip W Kuchel |
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Affiliation: | (1) Department of Biochemistry, University of Sydney, 2006 Sydney, N.S.W.;(2) Department of Clinical Biochemistry, Royal Prince Alfred Hospital, 2050 Sydney, N.S.W.;(3) Division of Nutrition and Dietetics, Faculty of Health Sciences, University of Newcastle, 2308 Newcastle, N.S.W., Australia |
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Abstract: | 31P nuclear magnetic resonance (NMR) spectroscopy was used to measure the rate of acyl transfer from phosphatidylcholine (lecithin,
PC) in whole plasma and in high density lipoprotein (HDL). Spectral deconvolution was used to resolve overlapping resonances
in the31P NMR spectra of the phospholipids. Mean values of the acyl group transfer rates from PC in plasma and HDL were 36 μmol L−1h−1 and 19 μmol L−1h−1, respectively. The reciprocal nature of the decrease in the spectral peak intensities of PC, compared to the increase in
the intensities of the lysolecithin (lysoPC) peaks, suggested a substrate/product relationship consistent with the action
of lecithin:cholesterol acyltransferase (LCAT), the enzyme responsible for the esterification of free cholesterol in plasma.
LCAT involvement was confirmed by measuring the cholesterol esterification rate based on the13C NMR spectra obtained on lipid extracts from plasma that had been incubated at 37°C. Within experimental error, the rate
of lysoPC formation in plasma was shown to be equal to that of cholesteryl ester formation. |
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