基于宏基因组的储粮害虫嗜卷书虱共生微生物多样性及功能分析

孤雌生殖的嗜卷书虱Liposcelis bostrychophila是储粮生态系统中易发害虫的优势种群之一，其共生微生物影响着嗜卷书虱L. bostrychophila的生物学特性。本文通过宏基因组测序技术和生物信息技术，分析了嗜卷书虱L. bostrychophila共生微生物的多样性及功能。结果表明：共鉴定出10门51属146种；变形菌门和厚壁菌门为菌群的优势门，平均相对丰度分别为90.05%和9.21%；立克次氏体属和葡萄球菌属为菌群的优势属，平均相对丰度分别为88.99%和9.03%。嗜卷书虱L. bostrychophila成虫体内微生物参与的碳水化合物代谢通路和复制与修复通路的丰度最高，平均相对丰度分别为9.0%和6.8%，表明嗜卷书虱L. bostrychophila体内共生微生物在宿主取食后协助碳水化合物的代谢，以及参与宿主部分DNA的复制和损失修复。本文系统揭示了嗜卷书虱L. bostrychophila共生微生物多样性及功能，以期能以共生微生物为切入点寻找储粮书虱的绿色防控提供有效途径。

Metagenomic insights into diversity, functions of symbiotic microorganisms of Liposcelis bostrychophila (Psocoptera: Liposcelididae) in the stored grain

Parthenogenetic Liposcelis bostrychophila is one of the dominant pest populations easily occurring in the grain storage ecosystem, and its symbiotic microorganisms affect the biological characteristics of the L. bostrychophila. In this paper, based on metagenomic sequencing technology and bioinformation technology, the diversity and function of the symbiotic microorganisms of L. bostrychophila were analyzed. The results showed that: 146 species symbiotic microorganisms of L. bostrychophila were identified from 51 genera of10 phyla. Proteobacteria and Firmicutes were the dominant phyla, with an average relative abundance of 90.05% and 9.21% respectively. Rickettsia and Staphylococcus were the dominant genera, with average relative abundance of 88.99% and 9.03% respectively. The abundance related to carbohydrate metabolism pathway and replication and repair pathway in L. bostrychophila adult were high, with an average relative abundance of 9.0% and 6.8% respectively. It indicated that the symbiotic microorganisms in bookworm assisted the metabolism of carbohydrate and participated in the replication and repair of partial DNA of the host after feeding. This paper systematically revealed the diversity and functions of the symbiotic microorganisms of L. bostrychophila in order to provide an effective way for the green control of the stored grain booklice.