| 阿魏酸荧光熄灭法测定痕量亚硝酸根 |
| |
| 引用本文： | 龙立平,姚丽娜,肖谷清,谢丹.阿魏酸荧光熄灭法测定痕量亚硝酸根[J].湖南城建高等专科学校学报,2010(4):48-51. |
| |
| 作者姓名： | 龙立平 姚丽娜 肖谷清 谢丹 |
| |
| 作者单位： | 湖南城市学院化学与环境工程系,湖南益阳413000 |
| |
| 基金项目： | 益阳市科技计划资助项目（20081416）; 湖南城市学院洞庭湖地区农副产品质量安全检测研究所资助项目 |
| |
| 摘 要： | 在pH 5.0的HAc～NaAc缓冲溶液中,阿魏酸与痕量亚硝酸根反应,使得阿魏酸在激发波长358.0nm,发射波长为450.0nm条件下的荧光强度明显下降,其相对荧光强度在一定范围内与亚硝酸根的浓度呈线性关系,从而建立了荧光熄灭法测定痕量亚硝酸根的新方法.方法的线性范围为1.00×10（-6）～8.00×10~（-3）mol/L,检出限为3.47×10~（-7）mol/L.方法已用于环境水样中痕量亚硝酸根的测定,回收率在96.7%～104.5%.
|
| 关 键 词： | 荧光光度法 荧光熄灭 阿魏酸 亚硝酸根 |
Determination of Trace Nitrite Based on Fluorescence Quenching with Ferulic Acid |
| |
| LONG Li-Ping,YAO Li-na,XIAO Gu-qing,XIE Dan.Determination of Trace Nitrite Based on Fluorescence Quenching with Ferulic Acid[J].Journal of Hunan Urban Construction College,2010(4):48-51. |
| |
| Authors: | LONG Li-Ping YAO Li-na XIAO Gu-qing XIE Dan |
| |
| Affiliation: | (College of Chemistry ＆ Environmental Engineering,Hunan City University,Yiyang,Hunan 413000,China) |
| |
| Abstract: | A new fluorescence quenching method was developed to determine nitrite in water samples. The method was based on the reaction between nitrite and ferulic acid in a buffer solution of HAc～NaAc（pH 5.0）.The fluorescence intensity was measured with excitation and emission wavelength of 358.0 and 450.0 nm, respectively.The relationship was obtained between the relation fluorescence intensity and nitrite concentration in the linear range from 1.00×10~（-6） mol/L～8.00×10~（-3） mol/L.The detection limit was 3.47×10~（-7） mol/L.It was applied to determine trace nitrite in water samples.The recoveries were 96.7%～104.5%. |
| |
| Keywords: | spectrophotofluorimetry fluorescence quench ferulic acid nitrite |
| 本文献已被 维普 等数据库收录！ |
