| 盐酸克伦特罗残留酶联免疫吸附(ELISA)检测方法的研究 |
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| 引用本文： | 陈存社,吕会田. 盐酸克伦特罗残留酶联免疫吸附(ELISA)检测方法的研究[J]. 食品与发酵工业, 2006, 32(7): 91-94 |
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| 作者姓名： | 陈存社 吕会田 |
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| 作者单位： | 北京工商大学化学与环境工程学院,北京,100037 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 建立了间接ELISA检测盐酸克伦特罗的方法,并对其参数进行了分析计算。在该检测方法中,抗盐酸克伦特罗(CL)抗体最适稀释度为1∶1000,羊抗兔酶联抗体(HRP-IgG)的最适稀释度为1∶1500。该检测方法的检测灵敏度可达0·1046μg/L,生物检测限为1·452μg/L,线性检测范围为7·26~90·75μg/L。
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| 关 键 词： | 盐酸克伦特罗 酶联免疫吸附检测(ELISA) 检测方法 |
| 收稿时间： | 2006-02-14 |
| 修稿时间： | 2006-05-11 |
The Enzyme-linked Immunosorbent Assay of Clenbuterol Residue |
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| Chen Cunshe,Lv Huitian. The Enzyme-linked Immunosorbent Assay of Clenbuterol Residue[J]. Food and Fermentation Industries, 2006, 32(7): 91-94 |
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| Authors: | Chen Cunshe Lv Huitian |
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| Affiliation: | School of Chemical and Environmental Engineering, Beijing Technology and Business University, Beijing 100037, China |
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| Abstract: | An indirect enzyme-linked immunosorbent assay(ELISA) was developed to detect and quantitate clenbuterol. The rabbit antiserum containing polyclonal antibodies against clenbuterol was prepared successfully by using BSA-CL as antigen. The antibody was purified with ammonium sulfate fractionation. The sensitivity of the assay is 0.104 6 μg/L and the biologic limit of detection was 1.452 μg/L, the standard curve was linear from 7.26 to 90.75 μg/L. |
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| Keywords: | clenbuterol hydrochloride enzyme-linked immunosorbent assay mensuration |
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