Kinetics of elimination and distribution in blood and liver of biocompatible ferrofluids based on Fe3O4 nanoparticles: An EPR and XRF study |
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| Authors: | L.F. Gamarra W.M. Pontuschka E. Amaro A.J. Costa-Filho G.E.S. Brito E.D. Vieira S.M. Carneiro D.M. Escriba A.M.F. Falleiros V.L. Salvador |
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| Affiliation: | 1. Instituto de Ensino e Pesquisa, Hospital Israelita Albert Einstein, São Paulo 05651-901, Brazil;2. Instituto de Física, Universidade de São Paulo, São Paulo 05315-970, Brazil;3. Instituto de Radiologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-001, Brazil;4. Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos 13560-970, Brazil;5. Laboratório de Biologia Celular, Instituto Butantan, São Paulo 05503-900, Brazil;6. Centro de Ciências Biológicas, Universidade Estadual de Londrina 86051-990, Brazil;7. Centro de aplicações e Lasers, IPEN, São Paulo 05508-000, Brazil |
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| Abstract: | In this study, we evaluated the biodistribution and the elimination kinetics of a biocompatible magnetic fluid, Endorem™, based on dextran-coated Fe3O4 nanoparticles endovenously injected into Winstar rats. The iron content in blood and liver samples was recorded using electron paramagnetic resonance (EPR) and X-ray fluorescence (XRF) techniques. The EPR line intensity at g = 2.1 was found to be proportional to the concentration of magnetic nanoparticles and the best temperature for spectra acquisition was 298 K. Both EPR and XRF analysis indicated that the maximum concentration of iron in the liver occurred 95 min after the ferrofluid administration. The half-life of the magnetic nanoparticles (MNP) in the blood was (11.6 ± 0.6) min measured by EPR and (12.6 ± 0.6) min determined by XRF. These results indicate that both EPR and XRF are very useful and appropriate techniques for the study of kinetics of ferrofluid elimination and biodistribution after its administration into the organism. |
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