阿特拉津微污染原水生物活性炭处理系统种群动态演替分析

采用降解菌柱、土壤悬液柱和对照柱3种活性炭柱处理阿特拉津微污染原水,考察了阿特拉津去除效率,并利用PCR-DGGE技术分析炭柱运行期间微生物菌群动态变化。结果表明140 d后对照柱去除率下降至30％～40％,而降解菌柱的去除效率保持在65%～75％。DGGE分析表明3个炭柱中都有自来水中微生物生长,土壤悬液柱中微生物在贫营养状态时种群多样性降低,实验室分离的阿特拉津降解菌接入降解菌柱后在运行期间可以保持相对优势,延长了炭的使用时间。

Analysis of microbial population dynamics in biological activated carbon system treating micro-polluted water by atrazine

The atrazine degrading-bacteria biological activated carbon column,soil-extract biological activated carbon column and the control（CK）column were designed to treat the micro-polluted water by atrazine at low concentration.The removal efficiency of atrazine was investigated and the microbial population dynamics was also analyzed by using the Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis（PCR-DGGE）technique.The results showed that the removal efficiency of atrazine in CK column fell to 30%—40％ while the degrading-bacteria column kept the efficiency around 65%—75% over 140 days operation.The pattern of DGGE showed that the atrazine-degrading bacteria isolated in the lab was relatively dominant after inoculation to the column during the operation which extended the life of the activated carbon.The microbial diversity in the soil-extract column decreased when operating in the oligotrophic condition and the microorganisms from tap water could grow on the three columns during operation.