| 固相萃取高效液相色谱法同时测定五仁醇浸膏中的5种木脂素 |
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| 引用本文： | 赵锦花,王治国,张小勇. 固相萃取高效液相色谱法同时测定五仁醇浸膏中的5种木脂素[J]. 延边大学学报(自然科学版), 2011, 0(2): 152-155 |
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| 作者姓名： | 赵锦花 王治国 张小勇 |
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| 作者单位： | [1]延边大学分析测试中心,吉林延吉133002 [2]延边大学理学院化学系,吉林延吉133002 |
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| 基金项目： | 吉林省科技发展计划项目资助（20100343） |
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| 摘 要： | 建立了五仁醇浸膏中5种木质素（五味子醇甲、五味子醇乙、五味子脂甲、五味子甲素和五味子乙素）的快速检测方法,并采用固相萃取高效液相色谱法对其进行了检测.结果显示：五味子醇甲、五味子醇乙、五味子脂甲、五味子甲素和五味子乙素进样量分别在0.048 8-2.44、0.040 8-2.04、0.049 6-2.48、0.036 0-1.80和0.048 0-2.40μg范围内具有良好的线性关系（R^2≤0.999 8）;平均加样回收率分别为101.24%、99.52%、103.72%、98.66%和98.08%,RSD≤1.62%.实验表明,固相萃取-高效液相色谱法具有简便、准确、稳定、灵敏度高、重现性好等优点,可用于五仁醇浸膏的质量控制.
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| 关 键 词： | 固相萃取 高效液相色谱法 五仁醇 木脂素 |
SPE-HPLC for Simultaneous Determination of Five Lignans from Nuts Alcohol |
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| ZHAO Jin-hua,WANG Zhi-guo,ZHANG Xiao-yong. SPE-HPLC for Simultaneous Determination of Five Lignans from Nuts Alcohol[J]. Journal of Yanbian University (Natural Science), 2011, 0(2): 152-155 |
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| Authors: | ZHAO Jin-hua WANG Zhi-guo ZHANG Xiao-yong |
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| Affiliation: | 1.Analysis and Inspection Center,Yanbian University,Yanji 133002,China;2.Department of Chemistry,College of Sciences,Yanbian University,Yanji 133002,China) |
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| Abstract: | A rapid and simple method based on solid phase extraction（SPE） combined with HPLC was developed and validated for simultaneous determination of five lignans（Schizandrin,Schizandrol B,Schizantherin A,Deoxyschizandrin and Schizandrin B） from Nuts Alcohol.The Schizandrin,Schizandrol B,Schizantherin A,Deoxyschizandrin and Schizandrin B were showed good linearity（R^2≤0.9998） within range of 0.0488-2.44,0.0408-2.04,0.0496-2.48,0.0360-1.80 and 0.0480-2.40μg,and the average recoveries were 101.24%,99.52%,103.72%,98.66% and 98.08% with low relative standard deviation（RSD≤1.62 %）,respectively.The results shown that the SPE-HPLC is a convenient,sensitive,robustness,reproducible and accurate protocol,it can be routinely used in the quality control of Nuts Alcohol. |
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| Keywords: | SPE HPLC nuts alcohol lignans |
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