Evaluation of ethanolysis with immobilized Candida antarctica lipase for regiospecific analysis of triacylglycerols containing highly unsaturated fatty acids |
| |
Authors: | Zhiping Shen Chakra Wijesundera |
| |
Affiliation: | (1) CSIRO Food Futures National Research Flagship, 3030 Werribee, Victoria, Australia;(2) Food Science Australia, 671 Sneydes Rd., 3030 Werribee, Victoria, Australia |
| |
Abstract: | The suitability of a recently proposed method based on ethanolysis with immobilized Candida antarctica lipase for regiospecific analysis of oils containing long-chain PUFA such as PA and DHA has been evaluated using selected
marine oils and regio-isomerically enriched synthetic TAG substrates. 1,3-Regios-electivity of the lipase was enhanced when
the ethanolysis was conducted in a high excess of ethanol, typically 10–50 times by weight of the oil. This enabled the reaction
to be conducted on a milligram scale. However, irrespective of the ethanol-to-oil ratio, C. antarctica lipase released FA from TAG at different rates depending on the degree of unsaturation and/or chain length of the FA. Differences
in lipolysis rates were particularly significant for EPA and DHA, with EPA released faster than DHA. Although DHA can be measured
with reasonable accuracy by ethanolysis with C. antarctica, the method requires further optimization before it can be adopted for reliable regiospecific analyses that are as accurate
as those obtainable by 13C NMR analysis for all major FA occurring in oils rich in long-chain PUFA. |
| |
Keywords: | Candida antarctica docosahexaenoic acid eicosapentaenoic acid FA composition lipase MAG marine oils 13C NMR regiospecific analysis TAG |
本文献已被 SpringerLink 等数据库收录! |
|