| 火焰子生物碱对酪氨酸酶的抑制作用 |
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| 引用本文： | 王晓梅,赵立芳,罗小林. 火焰子生物碱对酪氨酸酶的抑制作用[J]. 化学与生物工程, 2011, 28(1): 50-52. DOI: 10.3969/j.issn.1672-5425.2011.01.016 |
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| 作者姓名： | 王晓梅 赵立芳 罗小林 |
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| 作者单位： | 宝鸡文理学院化学化工系,陕西省植物化学重点实验室,陕西,宝鸡,721013 |
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| 基金项目： | 陕西省教育厅重点实验室基金资助项目，宝鸡文理学院院级重点项目 |
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| 摘 要： | 以磷酸缓冲溶液为反应体系、以左旋多巴为底物,采用分光光度法研究了火焰子生物碱对马铃薯酪氨酸酶活性的影响.结果表明,火焰子生物碱对马铃薯酪氨酸酶有抑制作用,抑制作用随着浓度的增大而增强,当火焰子生物碱浓度为1.25 mg·mL-1时,抑制率达最大,为29.27%,导致酶活力下降一半所需的抑制剂浓度(IC50)为0.93 ...
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| 关 键 词： | 火焰子 生物碱 酪氨酸酶 抑制 |
Inhibition of Alkaloids in Aconitum Sungpanense Hand.-mazz.on Tyrosinase Activity |
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| WANG Xiao-mei,ZHAO Li-fang,LUO Xiao-lin. Inhibition of Alkaloids in Aconitum Sungpanense Hand.-mazz.on Tyrosinase Activity[J]. Chemistry & Bioengineering, 2011, 28(1): 50-52. DOI: 10.3969/j.issn.1672-5425.2011.01.016 |
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| Authors: | WANG Xiao-mei ZHAO Li-fang LUO Xiao-lin |
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| Affiliation: | (Shanxi Province Key Laboratory of Phytochemistry,Department of Chemistry and Chemical Engineering,Baoji University of Arts and Sciences,Baoji 721013,China) |
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| Abstract: | With phosphate buffer as reaction system and L-dopa as substrate,inhibition on tyrosinase activity of alkaloids in Aconitum sungpanense hand.-mazz.(alkaloids in ASH)was studied by spectrophotometry.The results showed that alkaloids in ASH could inhibit tyrosinase activity,and inhibition effect increased with concentration of alkaloids in ASH.When the alkaloids concentration was 1.25 mg·mL-1,the inhibition rate reached 29.27%,IC50 was 0.93 mg·mL-1.The inhibition was reversible and non-competitive. |
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| Keywords: | Aconitum sungpanense hand.-mazz alkaloids tyrosinase inhibition |
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