| 响应面法优化醋酸菌的发酵产酸培养基 |
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| 引用本文： | 孙文瑛,陈雄,石勇,廖浩,申玉洁,雷锦成,李志军. 响应面法优化醋酸菌的发酵产酸培养基[J]. 化学与生物工程, 2011, 28(1): 37-41. DOI: 10.3969/j.issn.1672-5425.2011.01.013 |
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| 作者姓名： | 孙文瑛 陈雄 石勇 廖浩 申玉洁 雷锦成 李志军 |
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| 作者单位： | 1. 湖北工业大学生物工程学院,发酵工程省部共建教育部重点实验室,湖北,武汉,430068
2. 安琪酵母股份有限公司,湖北,宜昌,443003 |
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| 基金项目： | 湖北工业大学发酵工程省部共建教育部重点实验室2009年开放基金资助项目 |
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| 摘 要： | 参考相关文献,选择10种醋酸菌发酵培养基成分,依次利用Plackett-Burman实验、最陡爬坡实验和Box-Behnken实验进行培养基成分优化,证实乙醇、葡萄糖和谷氨酸钠对醋酸产量影响显著.通过回归拟合方程(R2=97.68%),预测当培养基成分配比为乙醇9.8%、葡萄糖2.7%、谷氨酸钠0.4%、酵母粉1%、乙...
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| 关 键 词： | 醋酸菌 响应面法 培养基 优化 |
Optimization of Fermentation Medium of Acetobacter Producing Acetic Acid Through Response Surface Method |
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| SUN Wen-ying,CHEN Xiong,SHI Yong,LIAO Hao,SHEN Yu-jie,LEI Jin-cheng,LI Zhi-jun. Optimization of Fermentation Medium of Acetobacter Producing Acetic Acid Through Response Surface Method[J]. Chemistry & Bioengineering, 2011, 28(1): 37-41. DOI: 10.3969/j.issn.1672-5425.2011.01.013 |
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| Authors: | SUN Wen-ying CHEN Xiong SHI Yong LIAO Hao SHEN Yu-jie LEI Jin-cheng LI Zhi-jun |
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| Affiliation: | 1.Key Laboratory of Fermentation Engineering of Ministry of Education,College of Bioengineering,Hubei University of Technology,Wuhan 430068,China;2.Angel Yeast Co.Ltd.,Yichang 443003,China) |
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| Abstract: | Ten ingredients of fermentation medium for acetobacter selected from references were optimized according to Plackett-Burman experiment,steepest ascent experiment and Box-Behnken experiment.It was found that ethanol,glucose and sodium glutamate affected the acetic aid production remarkably.A regression equation was obtained with credibility of 97.68% which predicted the optimum fermentation medium composition were as follows:ethanol 9.8%,glucose 2.7%,sodium glutamate 0.4%,yeast 1%,acetic acid 1%,peptone 0.2%,KH2PO4 0.1%,K2HPO4 0.1%,MgSO4·7H2O 0.02%,and the acetic acid production was 69.82 g·L-1.The model was proved to be feasible for predicting actual fermentation by three parallel validation tests. |
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| Keywords: | acetobacter response surface method medium optimizationo88 |
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