| 茶叶儿茶素组分HPLC测定中的提取方法研究 |
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| 引用本文： | 吕海鹏,林智,谷记平,郭丽,谭俊峰.茶叶儿茶素组分HPLC测定中的提取方法研究[J].食品与发酵工业,2007,33(6):76-79. |
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| 作者姓名： | 吕海鹏 林智 谷记平 郭丽 谭俊峰 |
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| 作者单位： | 1. 中国农业科学院茶叶研究所,农业部茶叶化学工程重点开放实验室,浙江杭州,310008
2. 中国农业科学院茶叶研究所,农业部茶叶化学工程重点开放实验室,浙江杭州,310008;中国科学院昆明植物所,植物化学与西部植物资源持续利用国家重点实验室,云南昆明,650204 |
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| 摘 要： | 以蒸青绿茶为材料,利用单因素试验和正交实验研究了不同提取时间、不同提取温度以及不同提取料液比例等对茶叶中儿茶素组分的影响。结果表明:当提取料液比例为1:125(g:mL)、提取温度为70℃、提取时间为30 min时,蒸馏水一次提取可以达到相对理想的提取效果,适用于常规分析实验。
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| 关 键 词： | 茶 儿茶素组分 高效液相色谱 提取方法 |
| 修稿时间： | 2007-03-152007-04-29 |
Study on Extracting Method of Catechins from Green Tea by HPLC |
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| Lv Haipeng,Lin Zhi,Gu Jiping,Guo Li,Tan Junfeng.Study on Extracting Method of Catechins from Green Tea by HPLC[J].Food and Fermentation Industries,2007,33(6):76-79. |
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| Authors: | Lv Haipeng Lin Zhi Gu Jiping Guo Li Tan Junfeng |
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| Affiliation: | 1.Key Laboratory of Tea Chemical Engineering , Ministry of Agriculture; Tea Research Institute , Chinese Academy of Agricultural Sciences , Hangzhou 310008,China;2.State Key Laboratory of Phytoehemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204,China |
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| Abstract: | The methods of extracting catechins from tea suitable for HPLC analysis were investigated with the orthogonal design tests as well as the single factor experiment.Results showed that the best extracted condition was as follows:lg of tea power sample,sifted through a 0.40mm sieve,infused by 125ml deionized water,and extracted once in 70℃water bath for 30min.This method was suitable for the routine determina- tion of catechins composition and content in tea.. |
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| Keywords: | tea catechins composition HPLC extracting method |
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