| 动物明胶中3种DNA提取方法的比较研究 |
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| 引用本文： | 陈颖,王晶,吴亚君,钱增敏,徐宝梁. 动物明胶中3种DNA提取方法的比较研究[J]. 食品与发酵工业, 2006, 32(4): 42-45 |
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| 作者姓名： | 陈颖 王晶 吴亚君 钱增敏 徐宝梁 |
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| 作者单位： | 中国检验检疫科学研究院,北京,100025 |
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| 基金项目： | 国家质量监督检验检疫总局科研项目 |
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| 摘 要： | 基于DNA水平的分子生物学检测方法,因其高特异性、高灵敏度且简便快速等优点,成为明胶动物来源鉴别检测的有效方法。但明胶的特殊加工工艺给DNA的提取带来了一定的困难。研究中选取了牛皮明胶、牛骨明胶和猪皮明胶等不同动物品种和不同动物组织来源的明胶产品,采用了试剂盒法、蛋白沉淀法和裂解抽提法等3种DNA提取方法,从DNA提取质量、提取效率及明胶动物种类来源PCR扩增等方面对3种方法进行了比较。研究结果表明,裂解抽提法所提取的明胶中的DNA质量优于前2种方法,且适合于进一步的PCR扩增检测,是适合于明胶DNA提取的有效方法。
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| 关 键 词： | 动物明胶 DNA提取 生物学检测 |
| 收稿时间： | 2005-11-21 |
| 修稿时间： | 2005-11-21 |
Comparison of Three DNA Extraction Methods from Animal Gelatins |
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| Chen Ying,Wang Jin,Wu Yajun,Qian Zengmin,Xu Baoliang. Comparison of Three DNA Extraction Methods from Animal Gelatins[J]. Food and Fermentation Industries, 2006, 32(4): 42-45 |
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| Authors: | Chen Ying Wang Jin Wu Yajun Qian Zengmin Xu Baoliang |
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| Affiliation: | Chinese Academy of Inspection and Quarantine, Beijing 100025, China |
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| Abstract: | Molecular biology method, based on DNA level, is an efficiency method for identification the animal source of gelatins duo to its high specificity, high sensitivity and high speed. However, the DNA extraction of gelatin is rather difficult because of its special processing. In this paper, three methods were applied for the DNA extraction of gelatins and gelatins which derived from different animals and different tissues were used as materials. After comparison with the DNA quality, DNA extraction efficiency and PCR detection result of these three methods, the lysis and extraction method was determined as suitable DNA extraction method for Gelatin. |
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| Keywords: | animal gelatin DNA extraction molecular biology method |
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