Feasibility of colloidal silver SERS for rapid bacterial screening |
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Authors: | Yongliang Liu Kuanglin Chao Xiangwu Nou Yud-Ren Chen |
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Affiliation: | (1) Food Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, USDA, Building 303, BARC-East, 10300 Baltimore Avenue, Beltsville, MD 20705, USA |
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Abstract: | This study reports the feasibility of citrate-reduced colloidal silver surface-enhanced Raman scattering (SERS) for differentiating
three important food borne pathogens, E. coli, Listeria, and Salmonella. FT-Raman and SERS spectra of both silver colloids and colloid-K3PO4 mixtures were collected and analyzed to evaluate the reproducibility and stability of silver colloids fabricated in a batch-production
process. The results suggest that the reproducibility of the colloids over the batch process is high and that their binding
effectiveness remains consistent over a 60-day storage period. Two specific SERS bands at 712 and 390 cm−1 were identified and used to develop simple 2-band ratios for differentiating E. coli-, Listeria-, and Salmonella-colloid mixtures with a 100% success. These results indicate that colloidal silver SERS technique may be a practical alternative
method suitable for routine and rapid screening of E. coli, Listeria, and Salmonella bacteria. |
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Keywords: | SERS Silver colloid E coli Listeria Salmonella Food safety |
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