Crystal structure of a synthetic cyclodecapeptide for template-assembled synthetic protein design |
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Authors: | Peluso S Rückle T Lehmann C Mutter M Peggion C Crisma M |
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Affiliation: | Institute of Organic Chemistry, University of Lausanne, BCH-Dorigny, 1015 Lausanne-Dorigny, Switzerland. |
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Abstract: | The structural prototype of a new generation of regioselectively addressable functionalized templates (RAFTs) for use in protein de novo design has been synthesized and crystallized. The structure of the aromatically substituted cyclodecapeptide was determined by X-ray diffraction; it consists of an antiparallel beta sheet spanned by heterochirally induced type IIprime prime or minute beta turns, similar to that observed in gramicidin S. The three-dimensional structure of the artificial template was also examined by an NMR spectroscopic analysis in solution and shown to be compatible with a beta-sheet plane suitable for accommodating secondary functional peptide fragments for the synthesis of template-assembled synthetic proteins (TASPs). |
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