| 一种测定红花籽粕中5-羟色胺衍生物总量的方法 |
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| 引用本文： | 王未鲜,金青哲,刘元法,王兴国. 一种测定红花籽粕中5-羟色胺衍生物总量的方法[J]. 中国油脂, 2006, 31(4): 50-52 |
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| 作者姓名： | 王未鲜 金青哲 刘元法 王兴国 |
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| 作者单位： | 江南大学食品学院,食品科学与安全教育部重点实验室,214036,江苏省无锡市惠河路170号 |
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| 摘 要： | 建立了一种以5-羟色胺硫酸肌酐为标准样品快速简便地测定红花籽粕5-羟色胺衍生物总量的方法。测定过程包括无水乙醚去油、甲醇回流提取5-羟色胺衍生物,再经过乙酸乙酯萃取,得到乙酸乙酯萃取物。根据5-羟色胺硫酸肌酐和Ehrlich试剂的反应来测定5-羟色胺衍生物的总量。得出的回归方程为y=0.0085 0.2213x。应用该法测定了4个样品,平均回收率为99.6%。实验表明该反应的选择性、重现性和对照性均好,操作方便,适用于5-羟色胺衍生物提取过程中的常规检测。
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| 关 键 词： | 红花籽粕 5-羟色胺 5-羟色胺衍生物 分光光度法 |
| 文章编号： | 1003-7969(2006)04-0050-03 |
| 收稿时间： | 2005-07-28 |
| 修稿时间： | 2005-07-28 |
Determination of total serotonin derivatives in safflower seed meal by spectrophotometry |
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| WANG Wei-xian,JIN Qing-zhe,LIU Yuan-fa,WANG Xing-guo. Determination of total serotonin derivatives in safflower seed meal by spectrophotometry[J]. China Oils and Fats, 2006, 31(4): 50-52 |
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| Authors: | WANG Wei-xian JIN Qing-zhe LIU Yuan-fa WANG Xing-guo |
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| Affiliation: | School of Food Science and Technology, Jiangnan University,214036 Jiangsu Wuxi, China |
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| Abstract: | A spectrophotometric method for determination of total serotonin derivatives was described.The method included a series of solvent extraction with diethyl ether anhydrous,methanol and ethyl acetate for the removal of oil and for the enrichment of serotonin.The final determination of serotonin was based on a color reaction between serotonin and Ehrlich's reagent.A regression equation y=0.0085 0.2213 x was obtained.The method was applied to four samples,with average recovery of 99.6%.Experimental results indicate that the method has good selectivity,reproducibility,and simplicity,and it is suitable for routine analysis. |
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| Keywords: | safflower seed meal serotonin serotonin derivatives spectrophotometry |
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