Cyclic fatty acid monomer: Isolation and purification with solid phase extraction |
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Authors: | Jose A Rojo Edward G Perkins |
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Affiliation: | (1) Department of Food Science, Burnsides Research Laboratory, University of Illinois, 1208 West Pennsylvania Avenue, 61801 Urbana, IL |
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Abstract: | The application of a combined solid phase extraction (SPE)/clean-up procedure to the isolation of a purified fraction containing
all the monomeric cyclic fatty acid methyl esters is described. Extraction of the nonpolar lipid components from nonurea-adducting
(NUA) filtrates is performed on a reverse phase octadecyl bonded silica minicolumn. Stepwise elution of the SPE-retained materials
through silica gel using several solvents allowed the separation of a pure fraction containing the cyclic monomers that can
be used for a more reliable quantitative estimation of these compounds in edible fats and oils. |
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