| 外源紫杉醇作用下南方红豆杉细胞凋亡及其动力学研究 |
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| 引用本文： | 王艳东,马振毅,元英进.外源紫杉醇作用下南方红豆杉细胞凋亡及其动力学研究[J].化学工程,2002,30(5):53-57. |
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| 作者姓名： | 王艳东 马振毅 元英进 |
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| 作者单位： | 天津大学,化工学院生物化工系,天津,300072 |
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| 基金项目： | 国家自然科学基金 (No .2 9976 0 32 )，教育部“跨世纪优秀人才培养计划”基金资助 |
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| 摘 要： | 利用光学显微照相技术、细胞总DNA琼脂糖凝胶电泳和TUNEL标记等手段 ,研究了外源紫杉醇(Taxol)作用下南方红豆杉细胞的凋亡及其动力学。研究结果表明 ,外源紫杉醇能显著提高南方红豆杉细胞凋亡的速率和发生率 ,表明高浓度紫杉醇不利于细胞的生长。凋亡动力学研究对指导大规模植物细胞培养生产紫杉醇具有重要意义
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| 关 键 词： | 紫杉醇 悬浮培养 南方红豆杉细胞 凋亡 凋亡动力学 |
| 文章编号： | 1005-9954(2002)05-0053-05 |
Studies on Apoptosis and Apoptotic Kinetics in Taxus Chinensis Var. Mairei Cells of Suspension Culture Treated with Taxol |
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| WANG Yan-dong,MA Zhen-yi,YUAN Ying-jin.Studies on Apoptosis and Apoptotic Kinetics in Taxus Chinensis Var. Mairei Cells of Suspension Culture Treated with Taxol[J].Chemical Engineering,2002,30(5):53-57. |
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| Authors: | WANG Yan-dong MA Zhen-yi YUAN Ying-jin |
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| Abstract: | Apoptosis and apoptotic kinetics of suspension culture Taxus chinensis var. mairei cells treated with taxol were studied. The morphology of cells under optical microscopy, DNA extracted from these cells on agarose gel electrophoresis and TUNEL analysis revealed that apoptosis was reinforced after cells were treated with taxol. High concentration of taxol affected the growth of cells observably. It was very important to increase the yield of taxol by the instruction of apoptotic kinetics. |
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| Keywords: | taxol suspension culture Taxus chinensis var Mairei apoptosis apoptotic kinetics |
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