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甜菜M14品系短期盐胁迫下生理生化指标研究
引用本文:贾姗姗,潘钰,马春泉,于冰,陈思学,李海英. 甜菜M14品系短期盐胁迫下生理生化指标研究[J]. 黑龙江水专学报, 2012, 3(1): 79-82
作者姓名:贾姗姗  潘钰  马春泉  于冰  陈思学  李海英
作者单位:黑龙江大学生命科学学院,哈尔滨150080;黑龙江大学黑龙江省普通高等学校分子生物学重点实验室,哈尔滨150080;黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150080
基金项目:国家自然科学基金项目(31071473;30871566); 黑龙江大学高层次人才(创新团队)支持计划(Hdtd2010-05)
摘    要:甜菜M14品系是带有白花甜菜第9号染色体的栽培甜菜单体附加系,具有抗逆、无融合生殖等优异特性。前期工作表明,M14的幼苗可以在400mmol/L NaCl浓度下良好的生长,最高胁迫浓度可达700mmol/LNaCl浓度。以甜菜M14品系的为研究材料,通过测定短时间下不同NaCl浓度(0、200、400mmol/L)胁迫下的甜菜M14品系的叶片质膜透性、MDA含量、脯氨酸等渗透性物质的变化规律,研究甜菜M14品系的生理生化反应,为解释M14品系的高抗盐能力奠定实验基础。

关 键 词:甜菜M14品系  盐胁迫  生理生化

Studies on physiological and biochemical indexes of sugar beet monosomic addition line M14 under short-term salt stress
JIA Shan-shan , PAN Yu , MA Chun-quan , YU Bing , CHEN Si-xue , LI Hai-ying. Studies on physiological and biochemical indexes of sugar beet monosomic addition line M14 under short-term salt stress[J]. Journal of Heilongjiang Hydraulic Engineering College, 2012, 3(1): 79-82
Authors:JIA Shan-shan    PAN Yu    MA Chun-quan    YU Bing    CHEN Si-xue    LI Hai-ying
Affiliation:a,b,c(Heilongjiang University a.College of Life Sciences;b.Key Laboratory of Molecular Biology,College of Heilongjiang Province;c.Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Harbin 150800,China)
Abstract:Sugar beet monosomic addition line M14 are monosomic addition lines of B.Vulgaris L.with an alien chromosome 9 of B.corolliflora Zoss which have many excellent properties such as apomixis and stress tolerance.The previous studies demonstrated that the seedlings of M14 can grow well under 400 mmol/L NaCl and the highest stress concentration arrived 700 mmol/L NaCl.In this study,M14 seedlings by determining the variation of membrane permeability were proposed,content of MDA and proline and other permeable material of M14 leaves.The seedlings of M14 were treated with different concentrations of NaCl salt stress(0,200,400 mmol/L) in a short time.Studies on physiological and biochemical responses of sugar beet M14 will lay a foundation of explaining high salt resistance ability of them.
Keywords:sugar beet monosomic addition line M14  salt stress  physiology and biochemistry
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