| 高糖化力菌种的筛选及诱变育种 |
| |
| 引用本文： | 杨丹丹,钱志伟,陈茂彬,李锐利,方尚玲.高糖化力菌种的筛选及诱变育种[J].中国酿造,2010(1). |
| |
| 作者姓名： | 杨丹丹 钱志伟 陈茂彬 李锐利 方尚玲 |
| |
| 作者单位： | 湖北工业大学,发酵工程省部共建教育部重点实验室,湖北,武汉,430068 |
| |
| 基金项目： | 湖北省自然科学基金项目资助 |
| |
| 摘 要： | 固体曲中精化酶能将淀粉水解为葡萄精,进而被微生物发酵生成酒精.以分离筛选出的1株产糖化酶的黑曲霉(酶活265.21U/g干曲)为出发菌,经紫外线及原生质体紫外线复合诱变育种,获得1株糖化酶活力较高、生产性能稳定的菌株,其酶活达到3367.79U/g干曲,比原始菌株提高1169.86%.
|
| 关 键 词： | 糖化酶 糖化力 黑曲霉 |
Screening and breeding of high activity glucoamylase strains |
| |
| YANG Dandan,QIAN Zhiwei,CHEN Maobin,LI Ruili,FANG Shangling.Screening and breeding of high activity glucoamylase strains[J].China Brewing,2010(1). |
| |
| Authors: | YANG Dandan QIAN Zhiwei CHEN Maobin LI Ruili FANG Shangling |
| |
| Affiliation: | YANG D,an,QIAN Zhiwei,CHEN Maobin,LI Ruili,FANG Shangling (Key Laboratory of Fermentation Engineering,Ministry of Education,College of Bioengineering,Hubei University of Technology,Wuhan 430068,China) |
| |
| Abstract: | Glucoamylase in koji can hydrolyzed starch to glucose and glucose can be used by microorganism to produce ethanol.A strain Aspergillus niger isolated from koji,whose glucoamylase activity was 265.21U/g dry koji,was selected as starting strain.A mutant strain with high glucoamylase activity was obtained after UV Mutation and protoplast mutagenesis and the glucoamylase activity was 3367.79 U/g dry medium,which was 1169.86% higher than the starting strain. |
| |
| Keywords: | glucoamylase saccharifying power Aspergillus niger |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
