| 大肠埃希O157:H7等致病菌多重PCR法快速检测试剂盒的研制 |
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| 引用本文： | 王文娟,赵宏坤. 大肠埃希O157:H7等致病菌多重PCR法快速检测试剂盒的研制[J]. 中国食品学报, 2011, 11(3) |
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| 作者姓名： | 王文娟 赵宏坤 |
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| 作者单位： | 1. 山东农业大学动物科技学院,山东泰安,271018
2. 山东农业大学动物科技学院,山东泰安271018;青岛农业大学食品科学与工程学院山东省肉类食品质量安全工程技术研究中心,山东青岛266109 |
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| 基金项目： | 国家"食品安全"科技支撑重大项目，国家肉鸡产业技术体系建设专项，山东省科技支撑项目 |
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| 摘 要： | 目的:研制一种能够同时快速检测大肠埃希O157:H7、志贺氏菌和致病性蜡样芽孢杆菌的三重PCR试剂盒.方法:以大肠埃希O157:H7的hlyAB基因、痢疾志贺氏菌的IpaH基因和致病性蜡样芽孢杆菌的hblA基因作为目的基因片段O157:H7、痢疾志贺氏菌和致病性蜡样芽孢杆菌的特异性抗原基因序列,分别设计1对引物,并对其反应条件进行优化,建立1种多重PCR检测试剂盒.结果:本试剂盒可准确检测出生肉和即食肉制品中的上述3种致病菌,O157:H7检出极限为19.8 cfu/mL,志贺氏菌检出极限为17 cfu/mL,蜡样芽孢杆菌检出极限为17.7 cfu/mL.可在5 h内完成全部反应过程,得出检测结果.结论:本试剂盒在理论和实际应用方面均具有优越性,能够同时检测上述3种病原菌,可用于食品及其原料的生物安全检测,也可用于兽医临床诊断.
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| 关 键 词： | 多重PCR 食品检测 大肠埃希O157:H7 志贺氏菌 致病性蜡样芽孢杆菌沙门氏菌 |
Study on Instant Detection Kit of Escherichia Coli O157:H7 and Other Pathogenic Bacteria by the Multiplex PCR |
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| Wang Wenjuan,Zhao Hongkun. Study on Instant Detection Kit of Escherichia Coli O157:H7 and Other Pathogenic Bacteria by the Multiplex PCR[J]. Journal of Chinese Institute of Food Science and Technology, 2011, 11(3) |
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| Authors: | Wang Wenjuan Zhao Hongkun |
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| Affiliation: | Wang Wenjuan1 Zhao Hongkun1,2 (1College of Animal Science and Veterinary Medicine,Shandong Agricultural University Tai'an 271018,Shandong 2College of Food Science and Engineering,Qingdao Agricultural University,Shandong Engineering Research Center for Meat Food Quality Safety,Qingdao 266109,Shandong) |
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| Abstract: | Objective:To develope a instant kit for detection of Escherichia coli O157:H7,Shigella spp.and Bacillus cereus by multiplexes PCR at the same time.Methods:Characteristic genes such as hlyAB,IpaH and hblA were chosen as the target genes for duplex PCR system.Many factors of duplex PCR were optimized.A multiplex PCR detection kit was established.Results:This kit can accurately detect those three pathogens both in raw and RTE meat products.The sensitivity was up to 19.8 cfu/mL of Escherichia coli O157:H7,17 cf... |
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| Keywords: | multiplex PCR food detection Escherichia coli O157:H7 Shigella spp. Bacillus cereus |
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