| 酶膜反应器制备燕麦蛋白质ACE抑制肽的研究 |
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| 引用本文： | 任清,张晓平. 酶膜反应器制备燕麦蛋白质ACE抑制肽的研究[J]. 中国食品学报, 2011, 11(4) |
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| 作者姓名： | 任清 张晓平 |
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| 作者单位： | 北京工商大学食品学院 食品添加剂与配料北京高校工程研究中心 北京100037 |
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| 摘 要： | 通过研究酶膜反应器系统对酶活的影响,可知酶膜反应器系统对碱性蛋白酶(Alcalase)具有很好的保留效果.蠕动泵运转过程对酶造成的破坏以及超滤膜对酶的吸附是导致碱性蛋白酶酶活损失的主要原因.通过利用Design Expert 6.0.5设计四因素三水平的响应面分析试验,求得酶膜反应器制备燕麦蛋白质ACE抑制肽的最佳工艺条件为底物质量分数3％、加酶量3.5％、料液流量45 L/h、操作压力0.07 MPa、温度61℃、pH 8,在该备件下产物的ACE抑制率可达67.86％.
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| 关 键 词： | 燕麦蛋白 酶膜反应器 ACE抑制肤 中空纤维膜 |
Study on Preparation of ACE Inhibitory Peptides from Oat Protein by Enzymatic Membrane Reactor |
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| Ren Oing,Zhang Xiaoping. Study on Preparation of ACE Inhibitory Peptides from Oat Protein by Enzymatic Membrane Reactor[J]. Journal of Chinese Institute of Food Science and Technology, 2011, 11(4) |
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| Authors: | Ren Oing Zhang Xiaoping |
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| Affiliation: | Ren Qing Zhang Xiaoping(Beijing Key Laboratory of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100037) |
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| Abstract: | By researching the influence of the enzymatic membrane reactor on enzyme activity,it was determined that the enzyme was perfectly confined in the enzymatic membrane reactor,and the destruction of pumping and adsorption of membrane were the main factors of the loss of enzyme activity. And by using Design Expert 6.0.5 to design response surface methodology of four factors and three levels,the optimum conditions for preparing ACE inhibitory peptides by hydrolysis of oat bran protein in the enzymatic membrane r... |
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| Keywords: | oat protein enzymatic membrane reactor ACE inhibitory peptides hollow fiber ultrafiltration membrane |
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