Human endothelial progenitor cell attachment to polysaccharide-based hydrogels: A pre-requisite for vascular tissue engineering |
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Authors: | Noélie-Brunehilde Thébaud Dorothée Pierron Reine Bareille Catherine Le Visage Didier Letourneur Laurence Bordenave |
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Affiliation: | (1) Inserm, U577, Bordeaux, F-33076, France;(2) University Victor Segalen Bordeaux 2, Bordeaux, F-33076, France;(3) Inserm, U698, Bio-ingénierie, CHU Xavier Bichat, F-75877 Paris Cedex 18, France |
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Abstract: | A hydrogel was prepared from polysaccharides (pullulan/dextran/fucoidan) and evaluated as a novel biomaterial for Endothelial
Progenitor Cell (EPC) culture. Using a cross-linking process with sodium trimetaphosphate in aqueous solution, homogeneous,
transparent and easy to handle gels were obtained with a water content higher than 90%. Circular scaffolds (6 mm diameter
and 2 mm thickness discs) were used for cell culture. Different types of EPCs were used: CD34+ derived ECs from cord blood
and two sorts of CD133+ derived ECs from human bone marrow, old (30 days) and young (4 days) cells. EPCs were characterised
as endothelial cells by immunofluorescent stainings for CD31 and Dil-Ac-LDL. CD133+ derived ECs from bone marrow were characterized
by RT-PCR for CD31, VE-cadherin and KDR. HSVECs (Human Saphenous Vein Endothelial Cells) were used as control cells. We evaluated
whether different kinds of EPCs could adhere on this novel hydrogel 4 h and 24 h after seeding, by a colorimetric quantitative
test. EPCs adhered to hydrogels in serum- free conditions with values being over than 80% for young CD133+ cells at 4 h and
24 h. This pullulan-based hydrogel could constitute a suitable support for vascular cell adhesion as a pre-requisite for vascular
tissue engineering. |
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