甘油分散含银溶胶的制备及抑菌性能研究

以AgNO_3为银源,无水甘油作为还原剂和分散介质,PVP-K30为稳定剂和模板剂,首次以加热搅拌法一步制备了含纳米银的无水甘油溶胶。以紫外-可见吸收光谱、X射线粉末衍射、透射电子显微镜等手段对所得溶胶和溶胶中的纳米银颗粒进行表征,考察了AgNO_3加入量、反应时间等因素对纳米银溶胶和银单质形貌的影响。结果表明,当投料质量比(m(甘油)∶m(AgNO_3)∶m(PVP-K30))为200∶(0.5~1.5)∶5,反应温度为110℃,反应时间为0.5~3h,可以获得稳定的纳米银甘油溶胶,其中银单质的颗粒分散性好,形貌近球形,粒径为纳米级别。以大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和蜡样芽胞杆菌作为研究对象,对所得含银溶胶的抗菌性进行研究,发现钠米银溶胶对4种目标菌均有很好的抑菌性,其中对枯草芽孢杆菌抑菌效果最好,且溶胶中银单质粒径越小,抑菌效果越好。

Synthesis and Antibacterial Property of Colloidal Silver Dispersed in Anhydrous Glycerin

An anhydrous glycerol colloid containing nano-silver was synthesized for the first time with heating and stirring method by using silver nitrate (AgNOa) as silver source,anhydrous glycerol as reducing agent and dispersion medium,and PVP-K30 as a stabilizer and template agent.The resulted colloid and the colloidal silver were characterized by ultraviolet-visible spectroscopy,X-ray powder diffraction and transmission electron microscopy.The effects of the amount of AgNO3 and reaction time to the colloid and the morphology of metallic silver were explored.The results showed that the stable silver glycerol colloid can be synthesized under the following conditions:m(glycerol) ∶ m(AgNOa) ∶ m(PVP-K30) =200 ∶ (0.5-1.5) ∶ 5,reaction temperature 110 ℃,reaction time 0.5-3 h.The silver particles in the suspension are subglobose and well dispersed,and their particle sizes are in nano-level.The antibacterial property of the silver colloid was investigated using Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Bacillus cereus.The result showed that the anhydrous glycerol colloid containing nano-silver has good antibacterial property to the four target bacteria,and displays the strongest antibacterial effect on Bacillus subtilis.On the other hand,it was proved that the silver particles with smaller sizes possess better antibacterial property.