ε-聚赖氨酸产生菌的筛选

改进了筛选ε-聚赖氨酸产生菌的方法。在加有复合抑菌剂的初筛平板上涂布土壤悬液,于28℃培养7 d后喷洒次甲基兰溶液显色,挑出周围形成透明圈的菌落,再次接种培养,7d后挖取菌落周围的琼脂块,利用文中设计的简易转移装置,将琼脂块中的水溶性成分转移到滤纸上,然后分别用茚三酮试剂和Dragendorff试剂检测,挑取对2种试剂都呈阳性的菌落,进一步摇瓶复筛,发现1株放线菌的发酵液中有ε-聚赖氨酸。经生化反应鉴定和分子生物学鉴定,确定该菌株为灰橙链霉菌(Streptomyces griseoaurantiacus)。

Isolating of a ε-Polylysine-producing Strain

In this paper,we improved the method for isolating actinomycete which producesε-polylysine. Soil samples was inoculated on plates containing complex bacteriostats and cultivated at 28℃.7 days later, methylene blue solution was sprayed on each plate.The actinomycete colonies forming halos were picked out and re-innoculated on new plates.After 7 days cultivation,small agar pieces surrounding each colony were cut.The secreted products in agar pieces were transferred to filter papers by using a simple device designed by us.The forming spots on filter papers were separately detected by dragendorff's reagent and ninhydrin re- agent.Colonies which were positive on both reagents were selected and further fermented by shaking flask culture.Finally,a strain of actinomycete was found to produceε-polylysine.The strain was identified as Streptomyces griseoaurantiacus.