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| 吸水链霉菌A03抗菌活性产物发酵条件的优化 | |
| 引用本文： | 杨秀芳,卢彩鸽,刘霆,刘伟成,裘季燕,王慧敏.吸水链霉菌A03抗菌活性产物发酵条件的优化[J].农药,2011,50(2). |
| 作者姓名： | 杨秀芳 卢彩鸽 刘霆 刘伟成 裘季燕 王慧敏 |
| 作者单位： | 1. 北京市农林科学院植物保护环境保护研究所,北京100097;中国农业大学植物病理学系,北京100094 2. 北京市农林科学院植物保护环境保护研究所,北京,100097 3. 中国农业大学植物病理学系,北京100094;北京农学院,北京102206 |
| 基金项目： | 北京市自然科学基金资助项目(6101001); 公益性行业(农业)科研专项(200903049-07); 北京市农林科学院项目(2010A006) |
| 摘 要： | 目的]吸水链霉菌A03是自主分离的高效拮抗菌株,其活性代谢产物对多种植物病原真菌具有强烈抑制作用;为了建立其高产发酵工艺,并为过程放大提供技术参数,利用正交设计和单因素试验对其发酵培养基和培养条件进行优化。结果]获得的最适发酵培养基配方(g/L):葡萄糖5,可溶性淀粉5,黄豆粉10,磷酸二氢钾0.5,碳酸钙1,七水硫酸镁0.1;最优培养条件:液体种子菌龄40 h,500 mL摇瓶装液量80 mL,接种量为体积分数12%,摇床转速180 r/min,培养温度28℃,发酵周期216 h;发酵液抑菌直径达3.04 cm。结论]成功建立了该菌株的小试发酵工艺,发酵水平提高了52.0%,发酵周期缩短30.77%。研究结果为其发酵代谢调控和过程放大提供了基本参数。 |
| 关 键 词： | 吸水链霉菌 抗菌活性产物 发酵培养基 培养条件 |
The Optimization of Fermentation Conditions for Antifungal Metabolite Production with Streptomyces hygroscopicus A03 |
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| YANG Xiu-fang,LU Cai-ge,LIU Ting,LIU Wei-cheng,QIU Ji-yan,WANG Hui-min.The Optimization of Fermentation Conditions for Antifungal Metabolite Production with Streptomyces hygroscopicus A03[J].Pesticides,2011,50(2). | |
| Authors: | YANG Xiu-fang LU Cai-ge LIU Ting LIU Wei-cheng QIU Ji-yan WANG Hui-min |
| Affiliation: | YANG Xiu-fang1,2,LU Cai-ge1,LIU Ting1,LIU Wei-cheng1,QIU Ji-yan1,WANG Hui-min2,3(1.Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China,2.Department of Plant Pathology,China Agricultural University,Beijing 100094,3.Beijing University of Agriculture,Beijing 102206,China) |
| Abstract: | Aims]Streptomyces hygroscopicus A03 is an antagonistic strain isolated by this research group.In previous studies,the active metabolite of the strain presented a significant inhibitory effect against many phytopathogenic fungi.In order to develop an efficient fermentation process for producing antifungal substances with Streptomyces hygroscopicus A03 and provide technical parameters for the further scale up of the process,the medium components and culture conditions were optimized with methods of orthogona... |
| Keywords: | Streptomyces hygroscopicus antifungal metabolite fermentation medium culture condition |
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