Directionally solidified biopolymer scaffolds: Mechanical properties and endothelial cell responses |
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Authors: | Nicholas W Meghri Amalie E Donius Benjamin W Riblett Elizabeth J Martin Alisa Morss Clyne Ulrike G K Wegst |
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Affiliation: | (1) Department of Biomedical Engineering, Johns Hopkins University, 207 Clark Hall, 3400 N. Charles Street, Baltimore, MD 21218, USA; |
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Abstract: | Vascularization is a primary challenge in tissue engineering. To achieve it in a tissue scaffold, an environment with the
appropriate structural, mechanical, and biochemical cues must be provided enabling endothelial cells to direct blood vessel
growth. While biochemical stimuli such as growth factors can be added through the scaffold material, the culture medium, or
both, a well-designed tissue engineering scaffold is required to provide the necessary local structural and mechanical cues.
As chitosan is a well-known carrier for biochemical stimuli, the focus of this study was on structure-property correlations,
to evaluate the effects of composition and processing conditions on the three-dimensional architecture and properties of freeze-cast
scaffolds; to establish whether freeze-east scaffolds are promising candidates as constructs promoting vascularization; and
to conduct initial tissue culture studies with endothelial cells on flat substrates of identical compositions as those of
the scaffolds to test whether these are biocompatible and promote cell attachment and proliferation. |
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