| 虾青素高产突变株的选育 |
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| 引用本文： | 徐学明,金征宇,吕玉华. 虾青素高产突变株的选育[J]. 食品与发酵工业, 2000, 26(5): 22-27 |
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| 作者姓名： | 徐学明 金征宇 吕玉华 |
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| 作者单位： | 1. 无锡轻工大学食品学院，无锡，214036
2. 上海农科院饲料检测所，上海，201106 |
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| 摘 要： | 阐述了法夫酵母中从葡萄糖到虾青素的整条生物合成途径。用亚硝基胍 (NTG)作为诱变剂 ,2 脱氧 D 葡萄糖 (2 DG)作为“筛子” ,通过 3轮诱变和一次自然分离 ,得到高产虾青素的菌株 4 2 6 ,其类胡萝卜素总量提高了 371 % ,为 7 0 7μg/mL ,虾青素的比率从 75 %提高到 79% ,并且其稳定性良好。对 2 DG和二苯胺作为筛子的作用机理也作了初步探讨。
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| 关 键 词： | 法夫酵母 虾青素 高产突变株 生物合成途径 NTG 2-DG |
| 修稿时间： | 1999-06-12 |
Studies on Astaxanthin-overproducing Mutants of Phaffia rhodozyma |
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| Xu Xueming Jin Zhengyu. Studies on Astaxanthin-overproducing Mutants of Phaffia rhodozyma[J]. Food and Fermentation Industries, 2000, 26(5): 22-27 |
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| Authors: | Xu Xueming Jin Zhengyu |
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| Abstract: | The whole pathway of the biosynthesis from glucose to astaxanthin by P. rhodozyma is presented in this paper. The astaxanthin overproducing mutant 4 26 was obtained by means of NTG and 2 DG treatment. Compared with the parental strain L 0, the yield of total carotenoids was improved from 1.5?μg/mL to 7.07?μg/mL by 371%, the percentage of astaxanthin was improved from 75% to 79%. The genetic stability was good after ten generation. As a screen, the mechanism of 2 DG and dephenylamine were also analyzed preliminarily. |
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| Keywords: | Phaffia rhodozyma astaxanthin overproducing mutants biosynthetic pathway NTG 2 DG |
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