| SPA的高效表达及其亲和介质制备条件的优化 |
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| 引用本文： | 金卫华,张三燕.SPA的高效表达及其亲和介质制备条件的优化[J].湖北化工,2012(2):87-89. |
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| 作者姓名： | 金卫华 张三燕 |
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| 作者单位： | [1]武汉东湖学院,湖北武汉430212 [2]江西农业大学,江西南昌330045 |
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| 摘 要： | 使用M9培养基,以大肠杆菌BL21为宿主菌、pET32a（＋）为载体,在15℃、IPTG浓度为0.8 mmol.L-1、150 r.min-1、诱导时间为4 d的条件下,SPA能够得到高效表达;在SPA∶Sepharose 4B=1∶1（质量体积比）、偶联时间为14 h时,SPA偶联率为89.67%,对IgG的纯化效果最好。
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| 关 键 词： | SPA 高效表达 亲和介质 偶联率 IgG纯化 |
High-Level Expression and Optimization of Preparation for Affinity Chromatography Medium of SPA |
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| JIN Wei-hua,ZHANG San-yan.High-Level Expression and Optimization of Preparation for Affinity Chromatography Medium of SPA[J].Hubei Chemical Industry,2012(2):87-89. |
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| Authors: | JIN Wei-hua ZHANG San-yan |
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| Affiliation: | 1. Wuhan Donghu College ,Wuhan 430212 ,China; 2. Jiangxi Agricultural University, Nanchang 330045, China) |
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| Abstract: | SPA had high-level expression in E. coli BL21 with pET32a（＋） as expression plasmid vector in M9 medium under the conditions as follows：temperature was 15 ℃ ,IPTG concentration was 0.8 mmol .L-1 , rotation speed was 150 r . min -1 ,induction time was 4 h. The coupling rate of SPA was 89.67% and it had the best purification efficiency for IgG when the coupling time was 14 h and the proportion of SPA and Sepharose 4B was 1 ： 1（mass-volume ratio）. |
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| Keywords: | SPA high-level expression affinity chromatography medium coupling rate IgG purification |
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